TY - JOUR
T1 - Zinc allocation to and within Arabidopsis halleri seeds
T2 - Different strategies of metal homeostasis in accessions under divergent selection pressure
AU - Babst-Kostecka, Alicja
AU - Przybyłowicz, Wojciech J.
AU - Seget, Barbara
AU - Mesjasz-Przybyłowicz, Jolanta
N1 - Funding Information:
This research was undertaken at the nuclear microprobe facility of Materials Research Department, iThemba Laboratory for Accelerator Based Sciences in South Africa. We thank A. Słomka and A. Barnabas for contribution in seed anatomy, and N. Mongwaketsi for assistance in performing microanalyses. This work was supported by the POWROTY/REINTEGRATION programme of the Foundation for Polish Science co‐financed by the European Union under the European Regional Development Fund (POIR.04.04.00‐00‐1D79/16‐01), the National Institute of Environmental and Health Sciences Superfund Research Program (Grant P42ES04940) at The University of Arizona, the Ministry of Science and Higher Education (Grants for Young Scientists and PhD Students 4604/E‐37/M/2015), and the statutory fund of the W. Szafer Institute of Botany, Polish Academy of Sciences. W.J. Przybyłowicz and J. Mesjasz‐Przybyłowicz are recipients of the South African National Research Foundation incentive grants No 114693 and 114694, respectively.
Funding Information:
This research was undertaken at the nuclear microprobe facility of Materials Research Department, iThemba Laboratory for Accelerator Based Sciences in South Africa. We thank A. S?omka and A. Barnabas for contribution in seed anatomy, and N. Mongwaketsi for assistance in performing microanalyses. This work was supported by the POWROTY/REINTEGRATION programme of the Foundation for Polish Science co-financed by the European Union under the European Regional Development Fund (POIR.04.04.00-00-1D79/16-01), the National Institute of Environmental and Health Sciences Superfund Research Program (Grant P42ES04940) at The University of Arizona, the Ministry of Science and Higher Education (Grants for Young Scientists and PhD Students 4604/E-37/M/2015), and the statutory fund of the W. Szafer Institute of Botany, Polish Academy of Sciences. W.J. Przyby?owicz and J. Mesjasz-Przyby?owicz are recipients of the South African National Research Foundation incentive grants No 114693 and 114694, respectively.
Publisher Copyright:
© 2020 The Authors. Plant-Environment Interactions published by New Phytologist and John Wiley & Sons Ltd.
PY - 2020/12
Y1 - 2020/12
N2 - Vegetative tissues of metal(loid)-hyperaccumulating plants are widely used to study plant metal homeostasis and adaptation to metalliferous soils, but little is known about these mechanisms in their seeds. We explored essential element allocation to Arabidopsis halleri seeds, a species that faces a particular trade-off between meeting nutrient requirements and minimizing toxicity risks. Combining advanced elemental mapping (micro-particle induced X-ray emission) with chemical analyses of plant and soil material, we investigated natural variation in Zn allocation to A. halleri seeds from non-metalliferous and metalliferous locations. We also assessed the tissue-level distribution and concentration of other nutrients to identify possible disorders in seed homeostasis. Unexpectedly, the highest Zn concentration was found in seeds of a non-metalliferous lowland location, whereas concentrations were relatively low in all other seed samples—including metallicolous ones. The abundance of other nutrients in seeds was unaffected by metalliferous site conditions. Our findings depict contrasting strategies of Zn allocation to A. halleri seeds: increased delivery at lowland non-metalliferous locations (a likely natural selection toward enhanced Zn-hyperaccumulation in vegetative tissues) versus limited translocation at metalliferous sites where external Zn concentrations are toxic for non-tolerant plants. Both strategies are worth exploring further to resolve metal homeostasis mechanisms and their effects on seed development and nutrition.
AB - Vegetative tissues of metal(loid)-hyperaccumulating plants are widely used to study plant metal homeostasis and adaptation to metalliferous soils, but little is known about these mechanisms in their seeds. We explored essential element allocation to Arabidopsis halleri seeds, a species that faces a particular trade-off between meeting nutrient requirements and minimizing toxicity risks. Combining advanced elemental mapping (micro-particle induced X-ray emission) with chemical analyses of plant and soil material, we investigated natural variation in Zn allocation to A. halleri seeds from non-metalliferous and metalliferous locations. We also assessed the tissue-level distribution and concentration of other nutrients to identify possible disorders in seed homeostasis. Unexpectedly, the highest Zn concentration was found in seeds of a non-metalliferous lowland location, whereas concentrations were relatively low in all other seed samples—including metallicolous ones. The abundance of other nutrients in seeds was unaffected by metalliferous site conditions. Our findings depict contrasting strategies of Zn allocation to A. halleri seeds: increased delivery at lowland non-metalliferous locations (a likely natural selection toward enhanced Zn-hyperaccumulation in vegetative tissues) versus limited translocation at metalliferous sites where external Zn concentrations are toxic for non-tolerant plants. Both strategies are worth exploring further to resolve metal homeostasis mechanisms and their effects on seed development and nutrition.
KW - Zn allocation
KW - adaptation
KW - facultative metallophyte
KW - homeostasis
KW - metal hyperaccumulation
KW - micro-PIXE
KW - pseudometallophyte
KW - seed
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U2 - 10.1002/pei3.10032
DO - 10.1002/pei3.10032
M3 - Article
AN - SCOPUS:85109573013
SN - 2575-6265
VL - 1
SP - 207
EP - 220
JO - Plant-Environment Interactions
JF - Plant-Environment Interactions
IS - 3
ER -