TY - JOUR
T1 - ZBP-89, a Kruppel-type zinc finger protein, inhibits cell proliferation
AU - Remington, Monica C.
AU - Tarlé, Susan A.
AU - Simon, Babette
AU - Merchant, Juanita L.
N1 - Funding Information:
J.L.M. is an investigator of the Howard Hughes Medical Institute. These studies were supported in part by a Public Health Service Grant to J.L.M. (DK45729). B.S. was supported by the German Research Foundation Si 383/3-1. Oligonucleotides were synthesized by the University of Michigan DNA synthesis core facility. The authors acknowledge the assistance of Claire Rogers in the performance of ¯ow cytometry analysis and Gail Kelsey in preparing the manuscript.
PY - 1997/8/18
Y1 - 1997/8/18
N2 - ZBP-89 is a Kruppel-type zinc finger transcription factor that binds to GC-rich sequences. Overexpression of this factor prevents EGF induction of the gastrin promoter; therefore, we postulated that ZBP-89 may modulate cellular proliferation. To test this hypothesis, ZBP-89 was overexpressed in immortalized (GH4) and malignant (AGS) cell lines. Growth parameters, e.g., 3H-thymidine, BrdU labeling, flow cytometry and ornithine decarboxylase promoter activity were analyzed. The results show that DNA synthesis is inhibited and progression to S phase is blocked in GH4 cells. Collectively, these studies demonstrate that ZBP-89 inhibits cellular proliferation at least in part through its ability to bind and repress ornithine deacarboxlyase promoter activity.
AB - ZBP-89 is a Kruppel-type zinc finger transcription factor that binds to GC-rich sequences. Overexpression of this factor prevents EGF induction of the gastrin promoter; therefore, we postulated that ZBP-89 may modulate cellular proliferation. To test this hypothesis, ZBP-89 was overexpressed in immortalized (GH4) and malignant (AGS) cell lines. Growth parameters, e.g., 3H-thymidine, BrdU labeling, flow cytometry and ornithine decarboxylase promoter activity were analyzed. The results show that DNA synthesis is inhibited and progression to S phase is blocked in GH4 cells. Collectively, these studies demonstrate that ZBP-89 inhibits cellular proliferation at least in part through its ability to bind and repress ornithine deacarboxlyase promoter activity.
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U2 - 10.1006/bbrc.1997.7119
DO - 10.1006/bbrc.1997.7119
M3 - Article
C2 - 9268691
AN - SCOPUS:0031577368
SN - 0006-291X
VL - 237
SP - 230
EP - 234
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -