TY - GEN
T1 - Widely-tunable single fiber laser OPO for multimodal microscopy
AU - Cromey, Benjamin
AU - Batjargal, Orkhongua
AU - Qin, Yukun
AU - Crystal, Sean
AU - Kieu, Khanh
N1 - Funding Information:
For this project was provided by The National Science Foundation (NSF) through a Graduate Research Fellowship (DGE-1143953) and NSF ECCS (1610048), the ARCS Foundation, and The National Institutes of Health (NIH) (1R01EB020605). The liver tissue sample was provided by Dr. Timothy Lee at the University of Nevada Las Vegas. The mouse brain tissue was provided by Dr. Arthur Rigel at the University of Arizona
Funding Information:
Funding for this project was provided by The National Science Foundation (NSF) through a Graduate Research Fellowship (DGE-1143953) and NSF ECCS (1610048), the ARCS Foundation, and The National Institutes of Health (NIH) (1R01EB020605).
Publisher Copyright:
© 2020 SPIE.
PY - 2020
Y1 - 2020
N2 - Raman microscopy is a key technique for biological imaging since it can provide valuable information about the chemical constituents of a sample without any labels. However, because two wavelengths are required for either CARS or SRS to occur, most Raman imaging set ups use multiple lasers with complicated synchronization requirements. In this presentation, we discuss the design and performance of a tunable Ytterbium-based fiber laser and an optical parametric oscillator for Raman microscopy. Our system uses a single laser that creates both pump and probe beams via nonlinear optical effects. Due to its reasonable high peak power, this laser system is a suitable light source for multimodal microscopy using both Raman and multiphoton imaging functionalities.
AB - Raman microscopy is a key technique for biological imaging since it can provide valuable information about the chemical constituents of a sample without any labels. However, because two wavelengths are required for either CARS or SRS to occur, most Raman imaging set ups use multiple lasers with complicated synchronization requirements. In this presentation, we discuss the design and performance of a tunable Ytterbium-based fiber laser and an optical parametric oscillator for Raman microscopy. Our system uses a single laser that creates both pump and probe beams via nonlinear optical effects. Due to its reasonable high peak power, this laser system is a suitable light source for multimodal microscopy using both Raman and multiphoton imaging functionalities.
KW - Fiber laser
KW - Multimodal microscopy
KW - Multiphoton microscopy
KW - OPO
KW - Stimulated raman scattering
UR - http://www.scopus.com/inward/record.url?scp=85084192820&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85084192820&partnerID=8YFLogxK
U2 - 10.1117/12.2546599
DO - 10.1117/12.2546599
M3 - Conference contribution
AN - SCOPUS:85084192820
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Nonlinear Frequency Generation and Conversion
A2 - Schunemann, Peter G.
A2 - Schepler, Kenneth L.
PB - SPIE
T2 - Nonlinear Frequency Generation and Conversion: Materials and Devices XIX 2020
Y2 - 3 February 2020 through 5 February 2020
ER -