Wide-field nano-scale imaging on a chip

Wide-field imaging facilitates the detection of rare events and reduces imaging time for large-area samples. Nano-scale imaging enables the detection and enumeration of individual nanoparticles and sub-cellular bioparticles. Uniting these two capabilities in a compact and field-portable on-chip imaging platform through lensfree holographic microscopy provides deep submicron resolution with high sensitivity over fields of view in the range 10-1800mm² - approximately 150-2700 times larger than a typical 40-215;objective field of view. High resolution is achieved via a pixel super-resolution approach, while high sensitivity is obtained via a sample preparation procedure that generates self-assembled nanolenses around individual nanoparticles and viruses. The foundations of these computational imaging approaches, along with their methodology and results, are discussed.