TY - JOUR
T1 - White spot syndrome virus (WSSV) prevalence in wild crustaceans in the Bohai Sea
AU - Xu, Tingting
AU - Shan, Xiujuan
AU - Li, Yingxia
AU - Yang, Tao
AU - Teng, Guangliang
AU - Wu, Qiang
AU - Wang, Chong
AU - Tang, Kathy F.J.
AU - Zhang, Qingli
AU - Jin, Xianshi
N1 - Funding Information:
The authors would like to thank Mr. Fangqun Dai and the staffs in the research vessel for them generous help in sampling. This work was supported by the National R&D Program of China ( 2017YFC1404503 ), Projects of International Exchange and Cooperation in Agriculture , Ministry of Agriculture and Rural Affairs (MARA) of China-Science, Technology and Innovation Cooperation in Aquaculture with Tropical Countries along the Belt and Road, Project of Species Conservation from the MARA-Marine fisheries resources collection and preservation, and Central Public-interest Scientific Institution Basal Research Fund, CAFS (NO. 2020TD39 ; 20603022020005 ).
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/9/15
Y1 - 2021/9/15
N2 - The impacts of mariculture on coastal ecosystems are increasingly raising attention recently. To study the prevalence of white spot syndrome virus (WSSV) in wild crustaceans, a pathogenic agent of cultured shrimps, continuous and large-scale surveys in the Bohai Sea were firstly conducted from 2016 to 2018. A total of 820 samples were collected from 59 sampling sites. The results of loop-mediated isothermal amplification (LAMP) assay showed that 11 of the 19 wild species were identified to be WSSV positive. Among them, Alpheus distinguendus had the highest WSSV-positive rate at 21.9%. The percentage of WSSV positive sampling sites was 76.7%, 55.0%, 43.7% in 2016, 2017, 2018, respectively. The percentage of WSSV positive samples was 17.4%, 12.2% and 7.8%, respectively. The multiple sequence alignment and phylogenetic tree analysis showed that the WSSV VP26 gene cloned from the wild crustaceans was consistent with those from WSSV strains previously reported in India, China, Brazil, Thailand, as well as Mexico, which was clustered tightly into one branch. The presence of WSSV virions in the epithelial cells of wild southern rough shrimp Trachypenaeus curvirostris was confirmed by TEM assay, which further verifying the occurrence of WSSV infection in wild crustaceans. The results of present study demonstrated that WSSV had colonized in the populations of offshore wild crustaceans in the Bohai Sea, and the potential negative impact of WSSV prevalence on the wild crustacean populations together with marine ecosystem deserved close attention and further investigation.
AB - The impacts of mariculture on coastal ecosystems are increasingly raising attention recently. To study the prevalence of white spot syndrome virus (WSSV) in wild crustaceans, a pathogenic agent of cultured shrimps, continuous and large-scale surveys in the Bohai Sea were firstly conducted from 2016 to 2018. A total of 820 samples were collected from 59 sampling sites. The results of loop-mediated isothermal amplification (LAMP) assay showed that 11 of the 19 wild species were identified to be WSSV positive. Among them, Alpheus distinguendus had the highest WSSV-positive rate at 21.9%. The percentage of WSSV positive sampling sites was 76.7%, 55.0%, 43.7% in 2016, 2017, 2018, respectively. The percentage of WSSV positive samples was 17.4%, 12.2% and 7.8%, respectively. The multiple sequence alignment and phylogenetic tree analysis showed that the WSSV VP26 gene cloned from the wild crustaceans was consistent with those from WSSV strains previously reported in India, China, Brazil, Thailand, as well as Mexico, which was clustered tightly into one branch. The presence of WSSV virions in the epithelial cells of wild southern rough shrimp Trachypenaeus curvirostris was confirmed by TEM assay, which further verifying the occurrence of WSSV infection in wild crustaceans. The results of present study demonstrated that WSSV had colonized in the populations of offshore wild crustaceans in the Bohai Sea, and the potential negative impact of WSSV prevalence on the wild crustacean populations together with marine ecosystem deserved close attention and further investigation.
KW - Mariculture
KW - Pathogenic
KW - Prevalence
KW - White spot syndrome virus (WSSV)
KW - Wild crustaceans
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U2 - 10.1016/j.aquaculture.2021.736810
DO - 10.1016/j.aquaculture.2021.736810
M3 - Article
AN - SCOPUS:85105430532
SN - 0044-8486
VL - 542
JO - Aquaculture
JF - Aquaculture
M1 - 736810
ER -