Virus entéricos en Ambientes Acuáticos: Métodos De Concentración y detección

Translated title of the contribution: Waterborne enteric viruses: Concentration and detection methods

Meybe Saavedra, Cerraf Tovar, Walter Q. Betancourt

Research output: Contribution to journalArticlepeer-review

Abstract

Human enteric viruses have been recognized as major infectious agents involved in numerous waterborne outbreaks worldwide. Detection of these pathogens in aquatic environments is therefore extremely important to safeguard public health. Current approaches for detection of waterborne enteric viruses involve three basic steps: primary concentration through membrane filtration, secondary concentration through centrifugation, and detection through molecular and cell culture methods. New strategies for concentration of viral particles and improvements in molecular detection methods have revealed not only the most frequent genotypes involved in human disease but also new and emerging waterborne viruses. Although the cell culture assay is the gold standard for isolation of waterborne infectious viruses, new quantitative molecular amplification assays are being more frequently used for detection of cultivable and noncultivable enteric viruses. The advantages and disadvantages of current and alternative detection methods are discussed in this paper.

Translated title of the contributionWaterborne enteric viruses: Concentration and detection methods
Original languageSpanish
Pages (from-to)260-265
Number of pages6
JournalInterciencia
Volume37
Issue number4
StatePublished - Apr 2012
Externally publishedYes

ASJC Scopus subject areas

  • General

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