Virulence of three distinct Cryptosporidium parvum isolates for healthy adults

Pablo C. Okhuysen; Cynthia L. Chappell; Joseph H. Crabb; Charles R. Sterling; Herbert L. DuPont

doi:10.1086/315033

Virulence of three distinct Cryptosporidium parvum isolates for healthy adults

Pablo C. Okhuysen
, Cynthia L. Chappell
, Joseph H. Crabb
, Charles R. Sterling
, Herbert L. DuPont

Research output: Contribution to journal › Article › peer-review

405 Scopus citations

Abstract

The infectivity of three Cryptosporidium parvum isolates (Iowa [calf], UCP [calf], and TAMU [horse]) of the C genotype was investigated in healthy adults. After exposure, volunteers recorded the number and form of stools passed and symptoms experienced. Oocyst excretion was assessed by immunofluorescence. The IC₅₀ differed among isolates: Iowa, 87 (SE, 19; 95% confidence interval [CI], 48.67-126); UCP, 1042 (SE, 1000; 95% CI, 0-3004); and TAMU, 9 oocysts (SE, 2.34; 95% CI, 4.46-13.65); TAMU versus Iowa, P = .002 or UCP, P = .019. Isolates also differed significantly (P = .045) in attack rate between TAMU (86%) and Iowa (52%) or UCP (59%). A trend toward a longer duration of diarrhea was seen for the TAMU (94.5 h) versus UCP (81.6 h) and Iowa (64.2 h) isolates. C. parvum isolates of the C genotype differ in their infectivity for humans.

Original language	English (US)
Pages (from-to)	1275-1281
Number of pages	7
Journal	Journal of Infectious Diseases
Volume	180
Issue number	4
DOIs	https://doi.org/10.1086/315033
State	Published - 1999

ASJC Scopus subject areas

General Medicine

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@article{f078643234924d59a901ebce7a3068b7,

title = "Virulence of three distinct Cryptosporidium parvum isolates for healthy adults",

abstract = "The infectivity of three Cryptosporidium parvum isolates (Iowa [calf], UCP [calf], and TAMU [horse]) of the C genotype was investigated in healthy adults. After exposure, volunteers recorded the number and form of stools passed and symptoms experienced. Oocyst excretion was assessed by immunofluorescence. The IC50 differed among isolates: Iowa, 87 (SE, 19; 95\% confidence interval [CI], 48.67-126); UCP, 1042 (SE, 1000; 95\% CI, 0-3004); and TAMU, 9 oocysts (SE, 2.34; 95\% CI, 4.46-13.65); TAMU versus Iowa, P = .002 or UCP, P = .019. Isolates also differed significantly (P = .045) in attack rate between TAMU (86\%) and Iowa (52\%) or UCP (59\%). A trend toward a longer duration of diarrhea was seen for the TAMU (94.5 h) versus UCP (81.6 h) and Iowa (64.2 h) isolates. C. parvum isolates of the C genotype differ in their infectivity for humans.",

author = "Okhuysen, \{Pablo C.\} and Chappell, \{Cynthia L.\} and Crabb, \{Joseph H.\} and Sterling, \{Charles R.\} and DuPont, \{Herbert L.\}",

note = "Funding Information: Grant Support: US Environmental Protection Agency (CR-819814 and CR-824759), National Institutes of Health (R01 AI 41735-01 and RR-02558), ImmuCell Corporation, and The Food and Drug Administration (FD-U-001621-01).",

year = "1999",

doi = "10.1086/315033",

language = "English (US)",

volume = "180",

pages = "1275--1281",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "4",

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AU - Okhuysen, Pablo C.

AU - Chappell, Cynthia L.

AU - Crabb, Joseph H.

AU - Sterling, Charles R.

AU - DuPont, Herbert L.

N1 - Funding Information: Grant Support: US Environmental Protection Agency (CR-819814 and CR-824759), National Institutes of Health (R01 AI 41735-01 and RR-02558), ImmuCell Corporation, and The Food and Drug Administration (FD-U-001621-01).

PY - 1999

Y1 - 1999

N2 - The infectivity of three Cryptosporidium parvum isolates (Iowa [calf], UCP [calf], and TAMU [horse]) of the C genotype was investigated in healthy adults. After exposure, volunteers recorded the number and form of stools passed and symptoms experienced. Oocyst excretion was assessed by immunofluorescence. The IC50 differed among isolates: Iowa, 87 (SE, 19; 95% confidence interval [CI], 48.67-126); UCP, 1042 (SE, 1000; 95% CI, 0-3004); and TAMU, 9 oocysts (SE, 2.34; 95% CI, 4.46-13.65); TAMU versus Iowa, P = .002 or UCP, P = .019. Isolates also differed significantly (P = .045) in attack rate between TAMU (86%) and Iowa (52%) or UCP (59%). A trend toward a longer duration of diarrhea was seen for the TAMU (94.5 h) versus UCP (81.6 h) and Iowa (64.2 h) isolates. C. parvum isolates of the C genotype differ in their infectivity for humans.

AB - The infectivity of three Cryptosporidium parvum isolates (Iowa [calf], UCP [calf], and TAMU [horse]) of the C genotype was investigated in healthy adults. After exposure, volunteers recorded the number and form of stools passed and symptoms experienced. Oocyst excretion was assessed by immunofluorescence. The IC50 differed among isolates: Iowa, 87 (SE, 19; 95% confidence interval [CI], 48.67-126); UCP, 1042 (SE, 1000; 95% CI, 0-3004); and TAMU, 9 oocysts (SE, 2.34; 95% CI, 4.46-13.65); TAMU versus Iowa, P = .002 or UCP, P = .019. Isolates also differed significantly (P = .045) in attack rate between TAMU (86%) and Iowa (52%) or UCP (59%). A trend toward a longer duration of diarrhea was seen for the TAMU (94.5 h) versus UCP (81.6 h) and Iowa (64.2 h) isolates. C. parvum isolates of the C genotype differ in their infectivity for humans.

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JO - Journal of Infectious Diseases

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Virulence of three distinct Cryptosporidium parvum isolates for healthy adults

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