Viradel method for detection of rota virus from seawater

Sagar M. Goyal; Charles P. Gerba

doi:10.1016/0166-0934(83)90080-0

Viradel method for detection of rota virus from seawater

Sagar M. Goyal, Charles P. Gerba

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

This report describes the use of a virus adsorption-elution (Viradel) technique for the concentration and detection of rotavirus from seawater. Simian rotavirus SA-11 was used as a model virus because it can be readily propagated and plaqued in vitro. The virus adsorbed optimally to fiberglass epoxy filters when seawater was adjusted to pH 3.5 and aluminum chloride added to a concentration of 1.0 mM. Adsorbed virus was eluted with 6% beef extract at pH 10.5 and was further concentrated to a smaller volume by a modification of the organic flocculation method. Using this method, in conjunction with an indirect immunofluorescence test, rotavirus was detected in sewage-contaminated seawater.

Original language	English (US)
Pages (from-to)	279-285
Number of pages	7
Journal	Journal of Virological Methods
Volume	7
Issue number	5-6
DOIs	https://doi.org/10.1016/0166-0934(83)90080-0
State	Published - Dec 1983

Keywords

rotavirus marine pollution concentration method human enteric viruses

ASJC Scopus subject areas

Virology

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10.1016/0166-0934(83)90080-0

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title = "Viradel method for detection of rota virus from seawater",

abstract = "This report describes the use of a virus adsorption-elution (Viradel) technique for the concentration and detection of rotavirus from seawater. Simian rotavirus SA-11 was used as a model virus because it can be readily propagated and plaqued in vitro. The virus adsorbed optimally to fiberglass epoxy filters when seawater was adjusted to pH 3.5 and aluminum chloride added to a concentration of 1.0 mM. Adsorbed virus was eluted with 6% beef extract at pH 10.5 and was further concentrated to a smaller volume by a modification of the organic flocculation method. Using this method, in conjunction with an indirect immunofluorescence test, rotavirus was detected in sewage-contaminated seawater.",

keywords = "rotavirus marine pollution concentration method human enteric viruses",

author = "Goyal, {Sagar M.} and Gerba, {Charles P.}",

note = "Funding Information: This study was supported in part by a grant (NASOAA-D00049) from the Office of Marine Pollution Assessment, NOAA, U.S. Department of Commerce, Rockville, MD. Secretarial assistance of Joan Gray is gratefully acknowledged.",

year = "1983",

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doi = "10.1016/0166-0934(83)90080-0",

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journal = "Journal of Virological Methods",

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T1 - Viradel method for detection of rota virus from seawater

AU - Goyal, Sagar M.

AU - Gerba, Charles P.

N1 - Funding Information: This study was supported in part by a grant (NASOAA-D00049) from the Office of Marine Pollution Assessment, NOAA, U.S. Department of Commerce, Rockville, MD. Secretarial assistance of Joan Gray is gratefully acknowledged.

PY - 1983/12

Y1 - 1983/12

N2 - This report describes the use of a virus adsorption-elution (Viradel) technique for the concentration and detection of rotavirus from seawater. Simian rotavirus SA-11 was used as a model virus because it can be readily propagated and plaqued in vitro. The virus adsorbed optimally to fiberglass epoxy filters when seawater was adjusted to pH 3.5 and aluminum chloride added to a concentration of 1.0 mM. Adsorbed virus was eluted with 6% beef extract at pH 10.5 and was further concentrated to a smaller volume by a modification of the organic flocculation method. Using this method, in conjunction with an indirect immunofluorescence test, rotavirus was detected in sewage-contaminated seawater.

AB - This report describes the use of a virus adsorption-elution (Viradel) technique for the concentration and detection of rotavirus from seawater. Simian rotavirus SA-11 was used as a model virus because it can be readily propagated and plaqued in vitro. The virus adsorbed optimally to fiberglass epoxy filters when seawater was adjusted to pH 3.5 and aluminum chloride added to a concentration of 1.0 mM. Adsorbed virus was eluted with 6% beef extract at pH 10.5 and was further concentrated to a smaller volume by a modification of the organic flocculation method. Using this method, in conjunction with an indirect immunofluorescence test, rotavirus was detected in sewage-contaminated seawater.

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

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ER -

Viradel method for detection of rota virus from seawater

Abstract

Keywords

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