Vaccination with parenteral toxoid B protects hamsters against lethal challenge with toxin A-negative, toxin B-positive clostridium difficile but does not prevent colonization

Farida Siddiqui; Jennifer R. O'Connor; Kristin Nagaro; Adam Cheknis; Susan P. Sambol; Gayatri Vedantam; Dale N. Gerding; Stuart Johnson

doi:10.1093/infdis/jir688

Vaccination with parenteral toxoid B protects hamsters against lethal challenge with toxin A-negative, toxin B-positive clostridium difficile but does not prevent colonization

Farida Siddiqui, Jennifer R. O'Connor, Kristin Nagaro, Adam Cheknis, Susan P. Sambol, Gayatri Vedantam, Dale N. Gerding, Stuart Johnson

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Toxin A has historically been regarded as the primary virulence determinant in Clostridium difficile infection, but naturally occurring toxin A-negative, toxin B-positive (A-/B+) C. difficile strains are known to be virulent. To determine the role of toxin B in these strains, we immunized hamsters with a toxoid prepared from purified toxin B to determine whether they would be protected from lethal challenge with an A-/B+ strain of C. difficile.

Original language	English (US)
Pages (from-to)	128-133
Number of pages	6
Journal	Journal of Infectious Diseases
Volume	205
Issue number	1
DOIs	https://doi.org/10.1093/infdis/jir688
State	Published - Jan 1 2012

ASJC Scopus subject areas

General Medicine

Access to Document

10.1093/infdis/jir688

Cite this

Siddiqui, F., O'Connor, J. R., Nagaro, K., Cheknis, A., Sambol, S. P., Vedantam, G., Gerding, D. N., & Johnson, S. (2012). Vaccination with parenteral toxoid B protects hamsters against lethal challenge with toxin A-negative, toxin B-positive clostridium difficile but does not prevent colonization. Journal of Infectious Diseases, 205(1), 128-133. https://doi.org/10.1093/infdis/jir688

Vaccination with parenteral toxoid B protects hamsters against lethal challenge with toxin A-negative, toxin B-positive clostridium difficile but does not prevent colonization. / Siddiqui, Farida; O'Connor, Jennifer R.; Nagaro, Kristin et al.
In: Journal of Infectious Diseases, Vol. 205, No. 1, 01.01.2012, p. 128-133.

Research output: Contribution to journal › Article › peer-review

Siddiqui, F, O'Connor, JR, Nagaro, K, Cheknis, A, Sambol, SP, Vedantam, G, Gerding, DN & Johnson, S 2012, 'Vaccination with parenteral toxoid B protects hamsters against lethal challenge with toxin A-negative, toxin B-positive clostridium difficile but does not prevent colonization', Journal of Infectious Diseases, vol. 205, no. 1, pp. 128-133. https://doi.org/10.1093/infdis/jir688

@article{43da81e744e64c9ab508f5a64a27407f,

title = "Vaccination with parenteral toxoid B protects hamsters against lethal challenge with toxin A-negative, toxin B-positive clostridium difficile but does not prevent colonization",

abstract = "Toxin A has historically been regarded as the primary virulence determinant in Clostridium difficile infection, but naturally occurring toxin A-negative, toxin B-positive (A-/B+) C. difficile strains are known to be virulent. To determine the role of toxin B in these strains, we immunized hamsters with a toxoid prepared from purified toxin B to determine whether they would be protected from lethal challenge with an A-/B+ strain of C. difficile.",

author = "Farida Siddiqui and O'Connor, {Jennifer R.} and Kristin Nagaro and Adam Cheknis and Sambol, {Susan P.} and Gayatri Vedantam and Gerding, {Dale N.} and Stuart Johnson",

note = "Funding Information: Financial support. This work was supported by the Veterans Administration Merit Review Research program (S. J., D. N. G.).",

year = "2012",

month = jan,

day = "1",

doi = "10.1093/infdis/jir688",

language = "English (US)",

volume = "205",

pages = "128--133",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Vaccination with parenteral toxoid B protects hamsters against lethal challenge with toxin A-negative, toxin B-positive clostridium difficile but does not prevent colonization

AU - Siddiqui, Farida

AU - O'Connor, Jennifer R.

AU - Nagaro, Kristin

AU - Cheknis, Adam

AU - Sambol, Susan P.

AU - Vedantam, Gayatri

AU - Gerding, Dale N.

AU - Johnson, Stuart

N1 - Funding Information: Financial support. This work was supported by the Veterans Administration Merit Review Research program (S. J., D. N. G.).

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Toxin A has historically been regarded as the primary virulence determinant in Clostridium difficile infection, but naturally occurring toxin A-negative, toxin B-positive (A-/B+) C. difficile strains are known to be virulent. To determine the role of toxin B in these strains, we immunized hamsters with a toxoid prepared from purified toxin B to determine whether they would be protected from lethal challenge with an A-/B+ strain of C. difficile.

AB - Toxin A has historically been regarded as the primary virulence determinant in Clostridium difficile infection, but naturally occurring toxin A-negative, toxin B-positive (A-/B+) C. difficile strains are known to be virulent. To determine the role of toxin B in these strains, we immunized hamsters with a toxoid prepared from purified toxin B to determine whether they would be protected from lethal challenge with an A-/B+ strain of C. difficile.

UR - http://www.scopus.com/inward/record.url?scp=84555187507&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84555187507&partnerID=8YFLogxK

U2 - 10.1093/infdis/jir688

DO - 10.1093/infdis/jir688

M3 - Article

C2 - 22124129

AN - SCOPUS:84555187507

SN - 0022-1899

VL - 205

SP - 128

EP - 133

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 1

ER -

Vaccination with parenteral toxoid B protects hamsters against lethal challenge with toxin A-negative, toxin B-positive clostridium difficile but does not prevent colonization

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this