V-ras H expression confers hormone-independent in vitro growth to LNCaP prostate carcinoma cells

H. James Voeller, George Wilding, Edward P. Gelmann

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

The LNCaP human prostate cancer cell line is dependent on androgen for in vitro growth. To discover genes that may be responsible for progression of prostate cancer from hormone dependence to hormone independence, we transfected LNCaP cells with expression vectors that contained either the v-rasH or c-rasH gene under the control of the cadmium (Cd2+)-inducible human metallothionein-IIA promoter. Numerous derivative cell lines were isolated which manifested inducible expression of rasH p21 protein when the cells were treated with Cd2+. None of the cell lines transfected with c-rasH were found to have an altered growth phenotype. Several derivative cell lines expressing inducible v-rasH manifested hormone-independent growth in culture when treated with 107 m Cd2+. Cd2+ induction of vrasH p21 was also shown to increase anchorageindependent colony formation of the v-rasH-expressing cell lines tested. Expression of a dominant mutated oncogene can change the hormone-dependent growth phenotype of prostate cancer cells.

Original languageEnglish (US)
Pages (from-to)209-216
Number of pages8
JournalMolecular Endocrinology
Volume5
Issue number2
DOIs
StatePublished - Feb 1991
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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