TY - JOUR
T1 - Utilization of renal slices to evaluate the efficacy of chelating agents for removing mercury from the kidney
AU - Keith, R. L.
AU - Setiarahardjo, I.
AU - Fernando, Q.
AU - Aposhian, H. V.
AU - Gandolfi, A. J.
PY - 1997/1/15
Y1 - 1997/1/15
N2 - Mercury is an environmental contaminant that preferentially accumulates in the kidney. It has been previously shown using proton-induced X-ray emission analysis that mercury (HgCl2) accumulated in precision-cut rabbit renal cortical slices. In this study, the efficacy of seven chelating agents for the removal of Hg from renal dices has been examined. Rabbits were injected with HgCl2 (10 mg/kg) and 3 h later kidneys were sliced, or renal slices were exposed in vitro to a mildly toxic concentration of HgCl2 (5 x 10-5 M, 4 h). The slices were then treated in vitro with 10 mM concentrations of EDTA, lipoic acid (LA), penicillamine (PA), glutathione (GSH), 1,4-dithiothreitol (DTT), DMSA, or DMPS. DMPS proved to be the most effective in mobilizing Hg from in vivo or in vitro HgCl2-exposed renal tissue (>85% of control after 3 h incubation). Relative efficacies for the seven agents were DMPS > DMSA, PA > DTT, GSH > LA, EDTA. The use of renal slices appears to be a useful in vitro tool for assessing the efficacy of chelating agents on mobilizing accumulated Hg from renal tissue.
AB - Mercury is an environmental contaminant that preferentially accumulates in the kidney. It has been previously shown using proton-induced X-ray emission analysis that mercury (HgCl2) accumulated in precision-cut rabbit renal cortical slices. In this study, the efficacy of seven chelating agents for the removal of Hg from renal dices has been examined. Rabbits were injected with HgCl2 (10 mg/kg) and 3 h later kidneys were sliced, or renal slices were exposed in vitro to a mildly toxic concentration of HgCl2 (5 x 10-5 M, 4 h). The slices were then treated in vitro with 10 mM concentrations of EDTA, lipoic acid (LA), penicillamine (PA), glutathione (GSH), 1,4-dithiothreitol (DTT), DMSA, or DMPS. DMPS proved to be the most effective in mobilizing Hg from in vivo or in vitro HgCl2-exposed renal tissue (>85% of control after 3 h incubation). Relative efficacies for the seven agents were DMPS > DMSA, PA > DTT, GSH > LA, EDTA. The use of renal slices appears to be a useful in vitro tool for assessing the efficacy of chelating agents on mobilizing accumulated Hg from renal tissue.
KW - Chelators
KW - Emission spectroscopy
KW - Kidney slices
KW - Metals
KW - Proton-induced X-ray emission
UR - http://www.scopus.com/inward/record.url?scp=0031018144&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031018144&partnerID=8YFLogxK
U2 - 10.1016/S0300-483X(96)03531-7
DO - 10.1016/S0300-483X(96)03531-7
M3 - Article
C2 - 9020508
AN - SCOPUS:0031018144
SN - 0300-483X
VL - 116
SP - 67
EP - 75
JO - Toxicology
JF - Toxicology
IS - 1-3
ER -