Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region

Meizhong Luo; Yeisoo Yu; Hyeran Kim; David A Kudrna; Yuichiro Itoh; Robert J. Agate; Esther Melamed; José L. Goicoechea; Jayson Talag; Christopher Mueller; Wenming Wang; Jennifer Currie; Nicholas B. Sisneros; Rod A. Wing; Arthur P. Arnold

doi:10.1016/j.ygeno.2005.09.005

Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region

Meizhong Luo
, Yeisoo Yu
, Hyeran Kim
, David A Kudrna
, Yuichiro Itoh
, Robert J. Agate
, Esther Melamed
, José L. Goicoechea
, Jayson Talag
, Christopher Mueller
, Wenming Wang
, Jennifer Currie
, Nicholas B. Sisneros
, Rod A. Wing
, Arthur P. Arnold

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome.

Original language	English (US)
Pages (from-to)	181-190
Number of pages	10
Journal	Genomics
Volume	87
Issue number	1
DOIs	https://doi.org/10.1016/j.ygeno.2005.09.005
State	Published - Jan 2006

Keywords

Androgen receptor
BAC library
Bird
Genomics
Zebra finch

ASJC Scopus subject areas

Genetics

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10.1016/j.ygeno.2005.09.005

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Luo, M., Yu, Y., Kim, H., Kudrna, D. A., Itoh, Y., Agate, R. J., Melamed, E., Goicoechea, J. L., Talag, J., Mueller, C., Wang, W., Currie, J., Sisneros, N. B., Wing, R. A., & Arnold, A. P. (2006). Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region. Genomics, 87(1), 181-190. https://doi.org/10.1016/j.ygeno.2005.09.005

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title = "Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region",

abstract = "The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG\_\_Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98\% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome.",

keywords = "Androgen receptor, BAC library, Bird, Genomics, Zebra finch",

author = "Meizhong Luo and Yeisoo Yu and Hyeran Kim and Kudrna, \{David A\} and Yuichiro Itoh and Agate, \{Robert J.\} and Esther Melamed and Goicoechea, \{Jos{\'e} L.\} and Jayson Talag and Christopher Mueller and Wenming Wang and Jennifer Currie and Sisneros, \{Nicholas B.\} and Wing, \{Rod A.\} and Arnold, \{Arthur P.\}",

year = "2006",

month = jan,

doi = "10.1016/j.ygeno.2005.09.005",

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AU - Luo, Meizhong

AU - Yu, Yeisoo

AU - Kim, Hyeran

AU - Kudrna, David A

AU - Itoh, Yuichiro

AU - Agate, Robert J.

AU - Melamed, Esther

AU - Goicoechea, José L.

AU - Talag, Jayson

AU - Mueller, Christopher

AU - Wang, Wenming

AU - Currie, Jennifer

AU - Sisneros, Nicholas B.

AU - Wing, Rod A.

AU - Arnold, Arthur P.

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AB - The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome.

KW - Androgen receptor

KW - BAC library

KW - Bird

KW - Genomics

KW - Zebra finch

UR - https://www.scopus.com/pages/publications/29344459588

UR - https://www.scopus.com/pages/publications/29344459588#tab=citedBy

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AN - SCOPUS:29344459588

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SP - 181

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JO - Genomics

JF - Genomics

IS - 1

ER -

Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region

Abstract

Keywords

ASJC Scopus subject areas

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