TY - JOUR
T1 - Use of two mcf-7 cell variants to evaluate the growth regulatory potential of estrogen-induced products
AU - Davidson, Nancy E.
AU - Bronzert, Diane A.
AU - Gelmann, Edward P.
AU - Lippman, Marc E.
AU - Chambon, Pierre
PY - 1986/4
Y1 - 1986/4
N2 - Two variants of the human estrogen-responsive breast cancer cell line MCF-7, were utilized to study the expression of an estrogen-induced gene, pS2, and an estrogen-induced Mr52,000 protein. One variant cell line, 113, is growth inhibited after chronic exposure to estrogen. Both the pS2 gene product and the Mr 52,000 protein were produced at maximal levels at a time when 113 growth was inhibited by estrogen. The variant cell line, LY2, selected for its resistance to the growth-inhibitory effects of the antiestrogen, LY117018, grew normally in the presence of this drug, although both pS2 expression and Mr52,000 protein production were inhibited. These results confirm that the pS2 gene and Mr 52,000 protein are estrogen-regulated elements, but the lack of correlation between their activities and variant cell growth suggests that they are not major autocrine growth-stimulatory agents.
AB - Two variants of the human estrogen-responsive breast cancer cell line MCF-7, were utilized to study the expression of an estrogen-induced gene, pS2, and an estrogen-induced Mr52,000 protein. One variant cell line, 113, is growth inhibited after chronic exposure to estrogen. Both the pS2 gene product and the Mr 52,000 protein were produced at maximal levels at a time when 113 growth was inhibited by estrogen. The variant cell line, LY2, selected for its resistance to the growth-inhibitory effects of the antiestrogen, LY117018, grew normally in the presence of this drug, although both pS2 expression and Mr52,000 protein production were inhibited. These results confirm that the pS2 gene and Mr 52,000 protein are estrogen-regulated elements, but the lack of correlation between their activities and variant cell growth suggests that they are not major autocrine growth-stimulatory agents.
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M3 - Article
C2 - 3948173
AN - SCOPUS:0022641791
SN - 0008-5472
VL - 46
SP - 1904
EP - 1908
JO - Cancer Research
JF - Cancer Research
ER -