Use of non-radioactively labeled DNA probes for the detection of a baculovirus from Penaeus monodon by in situ hybridization on fixed tissue

Bonnie T. Poulos, Jocelyne Mari, Jean Robert Bonami, Rita Redman, Donald V. Lightner

    Research output: Contribution to journalArticlepeer-review

    30 Scopus citations

    Abstract

    Clones isolated from a genomic library of the baculovirus PmSNPV (= MBV) were used to prepare DNA probes for detection of PmSNPV in shrimp tissue. Davidson's AFA fixed shrimp tissues were probed using DNA that had been labeled with the hapten, digoxigenin, by an in situ hybridization assay. The DNA probes readily distinguished PmSNPV-infected from uninfected shrimp tissue. The reactions were localized to the hepatopancreas and midgut, the sites where this virus is detected by standard histological methods. The probes did not react to shrimp infected with Baculovirus penaei (BP = PvSNPV).

    Original languageEnglish (US)
    Pages (from-to)187-193
    Number of pages7
    JournalJournal of Virological Methods
    Volume49
    Issue number2
    DOIs
    StatePublished - Sep 1994

    Keywords

    • DNA probe
    • In situ hybridization
    • MBV
    • PmSNPV
    • Shrimp baculovirus

    ASJC Scopus subject areas

    • Virology

    Fingerprint

    Dive into the research topics of 'Use of non-radioactively labeled DNA probes for the detection of a baculovirus from Penaeus monodon by in situ hybridization on fixed tissue'. Together they form a unique fingerprint.

    Cite this