Use of integrated cell culture-PCR to evaluate the effectiveness of poliovirus inactivation by chlorine

Felisa Blackmer; Kelly A. Reynolds; Charles P. Gerba; Ian L. Pepper

doi:10.1128/AEM.66.5.2267-2268.2000

Use of integrated cell culture-PCR to evaluate the effectiveness of poliovirus inactivation by chlorine

Felisa Blackmer, Kelly A. Reynolds, Charles P. Gerba, Ian L. Pepper

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Current standards, based on cell culture assay, indicate that poliovirus is inactivated by 0.5 mg of free chlorine per liter after 2 min; however, integrated cell culture-PCR detected viruses for up to 8 min of exposure to the same chlorine concentration, requiring 10 min for complete inactivation. Thus, the contact time for chlorine disinfection of poliovirus is up to five times greater than previously thought.

Original language	English (US)
Pages (from-to)	2267-2268
Number of pages	2
Journal	Applied and environmental microbiology
Volume	66
Issue number	5
DOIs	https://doi.org/10.1128/AEM.66.5.2267-2268.2000
State	Published - May 2000

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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abstract = "Current standards, based on cell culture assay, indicate that poliovirus is inactivated by 0.5 mg of free chlorine per liter after 2 min; however, integrated cell culture-PCR detected viruses for up to 8 min of exposure to the same chlorine concentration, requiring 10 min for complete inactivation. Thus, the contact time for chlorine disinfection of poliovirus is up to five times greater than previously thought.",

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AB - Current standards, based on cell culture assay, indicate that poliovirus is inactivated by 0.5 mg of free chlorine per liter after 2 min; however, integrated cell culture-PCR detected viruses for up to 8 min of exposure to the same chlorine concentration, requiring 10 min for complete inactivation. Thus, the contact time for chlorine disinfection of poliovirus is up to five times greater than previously thought.

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Use of integrated cell culture-PCR to evaluate the effectiveness of poliovirus inactivation by chlorine

Abstract

ASJC Scopus subject areas

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