Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS

Katsuyuki Nakano; Terutoshi Nakao; Karl H. Schram; William M. Hammargren; Thomas D. McClure; Michael Katz; Esklid Petersen

doi:10.1016/0009-8981(93)90181-3

Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS

Katsuyuki Nakano
, Terutoshi Nakao
, Karl H. Schram
, William M. Hammargren
, Thomas D. McClure
, Michael Katz
, Esklid Petersen

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Using boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), a method for the simultaneous determination of 12 urinary modified nucleosides has been developed. The RP-HPLC fractions were identified by gas chromatography/mass spectrometry analysis. The HPLC quantitation of urinary nucleoside levels before and after surgery of cancer patients suggested that urinary 5′-deoxy-5′-.

Original language	English (US)
Pages (from-to)	169-183
Number of pages	15
Journal	Clinica Chimica Acta
Volume	218
Issue number	2
DOIs	https://doi.org/10.1016/0009-8981(93)90181-3
State	Published - Sep 30 1993
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical

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10.1016/0009-8981(93)90181-3

Cite this

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title = "Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS",

abstract = "Using boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), a method for the simultaneous determination of 12 urinary modified nucleosides has been developed. The RP-HPLC fractions were identified by gas chromatography/mass spectrometry analysis. The HPLC quantitation of urinary nucleoside levels before and after surgery of cancer patients suggested that urinary 5′-deoxy-5′-.",

author = "Katsuyuki Nakano and Terutoshi Nakao and Schram, \{Karl H.\} and Hammargren, \{William M.\} and McClure, \{Thomas D.\} and Michael Katz and Esklid Petersen",

note = "Funding Information: This studyw as supportedb y a grant from PatriarchT akahitoM iki and the Churcho f PerfectL iberty,J apan(to K.N.) and a grantf romthe ArizonaD isease ControlR esearchC ommissio(nt o K.H.S.). The authorsth ankD r. T. Yasakaf or encouragemeanntd Prof. S. Oda and his collaboratorfos r the useo f instruments.",

year = "1993",

month = sep,

day = "30",

doi = "10.1016/0009-8981(93)90181-3",

language = "English (US)",

volume = "218",

pages = "169--183",

journal = "Clinica Chimica Acta",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS

AU - Nakano, Katsuyuki

AU - Nakao, Terutoshi

AU - Schram, Karl H.

AU - Hammargren, William M.

AU - McClure, Thomas D.

AU - Katz, Michael

AU - Petersen, Esklid

N1 - Funding Information: This studyw as supportedb y a grant from PatriarchT akahitoM iki and the Churcho f PerfectL iberty,J apan(to K.N.) and a grantf romthe ArizonaD isease ControlR esearchC ommissio(nt o K.H.S.). The authorsth ankD r. T. Yasakaf or encouragemeanntd Prof. S. Oda and his collaboratorfos r the useo f instruments.

PY - 1993/9/30

Y1 - 1993/9/30

N2 - Using boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), a method for the simultaneous determination of 12 urinary modified nucleosides has been developed. The RP-HPLC fractions were identified by gas chromatography/mass spectrometry analysis. The HPLC quantitation of urinary nucleoside levels before and after surgery of cancer patients suggested that urinary 5′-deoxy-5′-.

AB - Using boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), a method for the simultaneous determination of 12 urinary modified nucleosides has been developed. The RP-HPLC fractions were identified by gas chromatography/mass spectrometry analysis. The HPLC quantitation of urinary nucleoside levels before and after surgery of cancer patients suggested that urinary 5′-deoxy-5′-.

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DO - 10.1016/0009-8981(93)90181-3

M3 - Article

C2 - 7508341

AN - SCOPUS:0027329792

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VL - 218

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EP - 183

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 2

ER -

Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS

Abstract

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