Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS

Katsuyuki Nakano; Terutoshi Nakao; Karl H. Schram; William M. Hammargren; Thomas D. McClure; Michael Katz; Esklid Petersen

doi:10.1016/0009-8981(93)90181-3

Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS

Katsuyuki Nakano, Terutoshi Nakao, Karl H. Schram, William M. Hammargren, Thomas D. McClure, Michael Katz, Esklid Petersen

Pharmacy Practice and Science

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Using boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), a method for the simultaneous determination of 12 urinary modified nucleosides has been developed. The RP-HPLC fractions were identified by gas chromatography/mass spectrometry analysis. The HPLC quantitation of urinary nucleoside levels before and after surgery of cancer patients suggested that urinary 5′-deoxy-5′-.

Original language	English (US)
Pages (from-to)	169-183
Number of pages	15
Journal	Clinica Chimica Acta
Volume	218
Issue number	2
DOIs	https://doi.org/10.1016/0009-8981(93)90181-3
State	Published - Sep 30 1993

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical

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title = "Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS",

abstract = "Using boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), a method for the simultaneous determination of 12 urinary modified nucleosides has been developed. The RP-HPLC fractions were identified by gas chromatography/mass spectrometry analysis. The HPLC quantitation of urinary nucleoside levels before and after surgery of cancer patients suggested that urinary 5′-deoxy-5′-.",

author = "Katsuyuki Nakano and Terutoshi Nakao and Schram, {Karl H.} and Hammargren, {William M.} and McClure, {Thomas D.} and Michael Katz and Esklid Petersen",

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doi = "10.1016/0009-8981(93)90181-3",

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T1 - Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS

AU - Nakano, Katsuyuki

AU - Nakao, Terutoshi

AU - Schram, Karl H.

AU - Hammargren, William M.

AU - McClure, Thomas D.

AU - Katz, Michael

AU - Petersen, Esklid

N1 - Funding Information: This studyw as supportedb y a grant from PatriarchT akahitoM iki and the Churcho f PerfectL iberty,J apan(to K.N.) and a grantf romthe ArizonaD isease ControlR esearchC ommissio(nt o K.H.S.). The authorsth ankD r. T. Yasakaf or encouragemeanntd Prof. S. Oda and his collaboratorfos r the useo f instruments.

PY - 1993/9/30

Y1 - 1993/9/30

N2 - Using boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), a method for the simultaneous determination of 12 urinary modified nucleosides has been developed. The RP-HPLC fractions were identified by gas chromatography/mass spectrometry analysis. The HPLC quantitation of urinary nucleoside levels before and after surgery of cancer patients suggested that urinary 5′-deoxy-5′-.

AB - Using boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), a method for the simultaneous determination of 12 urinary modified nucleosides has been developed. The RP-HPLC fractions were identified by gas chromatography/mass spectrometry analysis. The HPLC quantitation of urinary nucleoside levels before and after surgery of cancer patients suggested that urinary 5′-deoxy-5′-.

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Urinary excretion of modified nucleosides as biological marker of RNA turnover in patients with cancer and AIDS

Abstract

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