Uptake of modified low-density lipoproteins alters actin distribution and locomotor forces in macrophages

Celina V. Zerbinatti, Robert W. Gore

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

It is postulated that macrophage-derived foam cells accumulate in the arterial wall because they lose the ability to migrate after excessive ingestion of modified forms of low-density lipoproteins (LDL). To assess changes in locomotor force generating capacity of foam cells, we measured isometric forces in J774A.1 macrophages after cholesterol loading with oxidized (Ox-LDL) or aggregated (Agg-LDL) LDL using a novel magnetic force transducer. Ox-LDL loading reduced the ability of J774A.1 macrophages to generate isometric forces by 50% relative to control cells. Changes in force frequency consistent with reduced motility were detected as well. Agg-LDL loading was also detrimental to J774A.1 motility but to a lesser extent than Ox-LDL. Ox-LDL loading significantly reduced total actin levels and induced changes in the F-actin to G-actin distribution, whereas Agg-LDL loaded cells had significantly increased levels of total actin. These data provide evidence that cholesterol loading and subsequent accumulation decreases macrophage motility by reducing the cells' force generating capacity and that Ox-LDL appears to be more effective than Agg-LDL in disrupting the locomotor machinery.

Original languageEnglish (US)
Pages (from-to)C555-C561
JournalAmerican Journal of Physiology - Cell Physiology
Volume284
Issue number2 53-2
DOIs
StatePublished - Feb 1 2003
Externally publishedYes

Keywords

  • Actin cytoskeleton
  • Cell force
  • Cell motility
  • J774A.1 macrophage

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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