TY - JOUR
T1 - Unique Cleavage Planes in Frozen Red Cell Membranes
AU - Weinstein, Ronald S.
AU - Clowes, Alexander W.
AU - Mcnutt, N. Scott
N1 - Funding Information:
Supported in part by National Cancer Institute Grant CA-07368 from the National Institutes of Health and U. S. Public Health Service Training Grant No. 5 TO1 GM 00192.
PY - 1970/9
Y1 - 1970/9
N2 - The Bullivant freeze-cleave technique has been modified to allow for the casting of replicas of the faces produced both above and below a single cleavage through frozen specimens. Analysis of replicas of paired fracture faces indicates that membrane faces normally revealed by cleaving are complementary and originate from the interior of the cell membrane. The thickness of the layers demonstrated by cleaving and heat-etching indicates that the potential cleavage plane from which membrane Face A and B are generated exists near the center of the membrane. The results provide physical evidence for the presence of a lipid bilayer within at least part of the red cell membrane.
AB - The Bullivant freeze-cleave technique has been modified to allow for the casting of replicas of the faces produced both above and below a single cleavage through frozen specimens. Analysis of replicas of paired fracture faces indicates that membrane faces normally revealed by cleaving are complementary and originate from the interior of the cell membrane. The thickness of the layers demonstrated by cleaving and heat-etching indicates that the potential cleavage plane from which membrane Face A and B are generated exists near the center of the membrane. The results provide physical evidence for the presence of a lipid bilayer within at least part of the red cell membrane.
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U2 - 10.3181/00379727-134-34973
DO - 10.3181/00379727-134-34973
M3 - Article
C2 - 4917656
AN - SCOPUS:0014851909
VL - 134
SP - 1195
EP - 1198
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
SN - 0037-9727
IS - 4
ER -