Unique Cleavage Planes in Frozen Red Cell Membranes

Ronald S. Weinstein; Alexander W. Clowes; N. Scott Mcnutt

doi:10.3181/00379727-134-34973

Unique Cleavage Planes in Frozen Red Cell Membranes

Ronald S. Weinstein, Alexander W. Clowes, N. Scott Mcnutt

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The Bullivant freeze-cleave technique has been modified to allow for the casting of replicas of the faces produced both above and below a single cleavage through frozen specimens. Analysis of replicas of paired fracture faces indicates that membrane faces normally revealed by cleaving are complementary and originate from the interior of the cell membrane. The thickness of the layers demonstrated by cleaving and heat-etching indicates that the potential cleavage plane from which membrane Face A and B are generated exists near the center of the membrane. The results provide physical evidence for the presence of a lipid bilayer within at least part of the red cell membrane.

Original language	English (US)
Pages (from-to)	1195-1198
Number of pages	4
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	134
Issue number	4
DOIs	https://doi.org/10.3181/00379727-134-34973
State	Published - Sep 1970
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Unique Cleavage Planes in Frozen Red Cell Membranes",

abstract = "The Bullivant freeze-cleave technique has been modified to allow for the casting of replicas of the faces produced both above and below a single cleavage through frozen specimens. Analysis of replicas of paired fracture faces indicates that membrane faces normally revealed by cleaving are complementary and originate from the interior of the cell membrane. The thickness of the layers demonstrated by cleaving and heat-etching indicates that the potential cleavage plane from which membrane Face A and B are generated exists near the center of the membrane. The results provide physical evidence for the presence of a lipid bilayer within at least part of the red cell membrane.",

author = "Weinstein, {Ronald S.} and Clowes, {Alexander W.} and Mcnutt, {N. Scott}",

note = "Funding Information: Supported in part by National Cancer Institute Grant CA-07368 from the National Institutes of Health and U. S. Public Health Service Training Grant No. 5 TO1 GM 00192. ",

year = "1970",

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T1 - Unique Cleavage Planes in Frozen Red Cell Membranes

AU - Weinstein, Ronald S.

AU - Clowes, Alexander W.

AU - Mcnutt, N. Scott

N1 - Funding Information: Supported in part by National Cancer Institute Grant CA-07368 from the National Institutes of Health and U. S. Public Health Service Training Grant No. 5 TO1 GM 00192.

PY - 1970/9

Y1 - 1970/9

N2 - The Bullivant freeze-cleave technique has been modified to allow for the casting of replicas of the faces produced both above and below a single cleavage through frozen specimens. Analysis of replicas of paired fracture faces indicates that membrane faces normally revealed by cleaving are complementary and originate from the interior of the cell membrane. The thickness of the layers demonstrated by cleaving and heat-etching indicates that the potential cleavage plane from which membrane Face A and B are generated exists near the center of the membrane. The results provide physical evidence for the presence of a lipid bilayer within at least part of the red cell membrane.

AB - The Bullivant freeze-cleave technique has been modified to allow for the casting of replicas of the faces produced both above and below a single cleavage through frozen specimens. Analysis of replicas of paired fracture faces indicates that membrane faces normally revealed by cleaving are complementary and originate from the interior of the cell membrane. The thickness of the layers demonstrated by cleaving and heat-etching indicates that the potential cleavage plane from which membrane Face A and B are generated exists near the center of the membrane. The results provide physical evidence for the presence of a lipid bilayer within at least part of the red cell membrane.

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JO - Proceedings of the Society for Experimental Biology and Medicine

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Unique Cleavage Planes in Frozen Red Cell Membranes

Abstract

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