Ultrastructure of tight junctions in prostaglandin-exposed rat stomach

Ronald S. Weinstein; Barbara F. Banner; Jerome R. Kuszak; Neil J. Thomas; Bendicht U. Pauli

doi:10.1007/BF01309334

Ultrastructure of tight junctions in prostaglandin-exposed rat stomach

Ronald S. Weinstein, Barbara F. Banner, Jerome R. Kuszak, Neil J. Thomas, Bendicht U. Pauli

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The effect of intragastric misoprostol, a synthetic prostaglandin E₁ methyl ester analog, on the morphology of rat stomach was studied by transmission and scanning electron microscopy. Adult male Sprague-Dawley rats received misoprostol at dose levels ranging from 50 to 1000 μg/kg body weight and were sacrificed after 30 min. At all dose levels, normal organelle ultrastructure was maintained in epithelial cells on the surface, within gastric pits, and lining the gastric glands. Quantitative freeze-fracture electron microscopy was used to examine the influence of misoprostol on epithelial-cell tight junctions, major structural components of the mucosal barrier. The numbers of intramembrane fibrils and the depths of the tight-junction complexes did not differ significantly between controls and animals exposed to 1000 μg/kg misoprostol (P<0.05). Therefore, alterations in gastric epithelial tight-junction ultrastructure do not appear to account for the cytoprotective effect of misoprostol.

Original language	English (US)
Pages (from-to)	115S-119S
Journal	Digestive diseases and sciences
Volume	31
Issue number	2 Supplement
DOIs	https://doi.org/10.1007/BF01309334
State	Published - Feb 1986
Externally published	Yes

ASJC Scopus subject areas

Physiology
Gastroenterology

Access to Document

10.1007/BF01309334

Cite this

@article{cd527c8903414c7c9645c79aa5059fc4,

title = "Ultrastructure of tight junctions in prostaglandin-exposed rat stomach",

abstract = "The effect of intragastric misoprostol, a synthetic prostaglandin E1 methyl ester analog, on the morphology of rat stomach was studied by transmission and scanning electron microscopy. Adult male Sprague-Dawley rats received misoprostol at dose levels ranging from 50 to 1000 μg/kg body weight and were sacrificed after 30 min. At all dose levels, normal organelle ultrastructure was maintained in epithelial cells on the surface, within gastric pits, and lining the gastric glands. Quantitative freeze-fracture electron microscopy was used to examine the influence of misoprostol on epithelial-cell tight junctions, major structural components of the mucosal barrier. The numbers of intramembrane fibrils and the depths of the tight-junction complexes did not differ significantly between controls and animals exposed to 1000 μg/kg misoprostol (P<0.05). Therefore, alterations in gastric epithelial tight-junction ultrastructure do not appear to account for the cytoprotective effect of misoprostol.",

author = "Weinstein, {Ronald S.} and Banner, {Barbara F.} and Kuszak, {Jerome R.} and Thomas, {Neil J.} and Pauli, {Bendicht U.}",

year = "1986",

month = feb,

doi = "10.1007/BF01309334",

language = "English (US)",

volume = "31",

pages = "115S--119S",

journal = "Digestive diseases and sciences",

issn = "0163-2116",

publisher = "Plenum Publishers",

number = "2 Supplement",

}

TY - JOUR

T1 - Ultrastructure of tight junctions in prostaglandin-exposed rat stomach

AU - Weinstein, Ronald S.

AU - Banner, Barbara F.

AU - Kuszak, Jerome R.

AU - Thomas, Neil J.

AU - Pauli, Bendicht U.

PY - 1986/2

Y1 - 1986/2

N2 - The effect of intragastric misoprostol, a synthetic prostaglandin E1 methyl ester analog, on the morphology of rat stomach was studied by transmission and scanning electron microscopy. Adult male Sprague-Dawley rats received misoprostol at dose levels ranging from 50 to 1000 μg/kg body weight and were sacrificed after 30 min. At all dose levels, normal organelle ultrastructure was maintained in epithelial cells on the surface, within gastric pits, and lining the gastric glands. Quantitative freeze-fracture electron microscopy was used to examine the influence of misoprostol on epithelial-cell tight junctions, major structural components of the mucosal barrier. The numbers of intramembrane fibrils and the depths of the tight-junction complexes did not differ significantly between controls and animals exposed to 1000 μg/kg misoprostol (P<0.05). Therefore, alterations in gastric epithelial tight-junction ultrastructure do not appear to account for the cytoprotective effect of misoprostol.

AB - The effect of intragastric misoprostol, a synthetic prostaglandin E1 methyl ester analog, on the morphology of rat stomach was studied by transmission and scanning electron microscopy. Adult male Sprague-Dawley rats received misoprostol at dose levels ranging from 50 to 1000 μg/kg body weight and were sacrificed after 30 min. At all dose levels, normal organelle ultrastructure was maintained in epithelial cells on the surface, within gastric pits, and lining the gastric glands. Quantitative freeze-fracture electron microscopy was used to examine the influence of misoprostol on epithelial-cell tight junctions, major structural components of the mucosal barrier. The numbers of intramembrane fibrils and the depths of the tight-junction complexes did not differ significantly between controls and animals exposed to 1000 μg/kg misoprostol (P<0.05). Therefore, alterations in gastric epithelial tight-junction ultrastructure do not appear to account for the cytoprotective effect of misoprostol.

UR - http://www.scopus.com/inward/record.url?scp=0022622917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022622917&partnerID=8YFLogxK

U2 - 10.1007/BF01309334

DO - 10.1007/BF01309334

M3 - Article

C2 - 3080281

AN - SCOPUS:0022622917

SN - 0163-2116

VL - 31

SP - 115S-119S

JO - Digestive diseases and sciences

JF - Digestive diseases and sciences

IS - 2 Supplement

ER -

Ultrastructure of tight junctions in prostaglandin-exposed rat stomach

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this