TY - JOUR
T1 - Ultrastructure of the replication site in Taura syndrome virus (TSV)-infected cells
AU - Srisuvan, Thinnarat
AU - Pantoja, Carlos R.
AU - Redman, Rita M.
AU - Lightner, Donald V.
PY - 2006/12/14
Y1 - 2006/12/14
N2 - Taura syndrome virus (TSV) is a member of the family Dicistroviridae that infects Pacific white shrimp Litopenaeus vannamei (also called Penaeus vannamei), and its replication strategy is largely unknown. To identify the viral replication site within infected shrimp cells, the viral RNA was located in correlation with virus-induced membrane rearrangement. Ultrastructural changes in the infected cells, analyzed by transmission electron microscopy (TEM), included the induction and proliferation of intracellular vesicle-like membranes, while the intracytoplasmic inclusion bodies and pyknotic nuclei indicative of TSV infection were frequently seen. TSV plus-strand RNA, localized by electron microscopic in situ hybridization (EM-ISH) using TSV-specific cDNA probes, was found to be associated with the membranous structures. Moreover, TSV particles were observed in infected cells by TEM, and following EM-ISH, they were also seen in close association with the proliferating membranes. Taken together, our results suggest that the membranous vesicle-like structures carry the TSV RNA replication complex and that they are the site of nascent viral RNA synthesis. Further investigations on cellular origins and biochemical compositions of these membranous structures will elucidate the morphogenesis and propagation strategy of TSV.
AB - Taura syndrome virus (TSV) is a member of the family Dicistroviridae that infects Pacific white shrimp Litopenaeus vannamei (also called Penaeus vannamei), and its replication strategy is largely unknown. To identify the viral replication site within infected shrimp cells, the viral RNA was located in correlation with virus-induced membrane rearrangement. Ultrastructural changes in the infected cells, analyzed by transmission electron microscopy (TEM), included the induction and proliferation of intracellular vesicle-like membranes, while the intracytoplasmic inclusion bodies and pyknotic nuclei indicative of TSV infection were frequently seen. TSV plus-strand RNA, localized by electron microscopic in situ hybridization (EM-ISH) using TSV-specific cDNA probes, was found to be associated with the membranous structures. Moreover, TSV particles were observed in infected cells by TEM, and following EM-ISH, they were also seen in close association with the proliferating membranes. Taken together, our results suggest that the membranous vesicle-like structures carry the TSV RNA replication complex and that they are the site of nascent viral RNA synthesis. Further investigations on cellular origins and biochemical compositions of these membranous structures will elucidate the morphogenesis and propagation strategy of TSV.
KW - In situ hybridization
KW - Litopenaeus vannamei
KW - TSV
KW - Taura syndrome virus
KW - Transmission electron microscopy
UR - http://www.scopus.com/inward/record.url?scp=33846430004&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846430004&partnerID=8YFLogxK
U2 - 10.3354/dao073089
DO - 10.3354/dao073089
M3 - Article
C2 - 17260828
AN - SCOPUS:33846430004
SN - 0177-5103
VL - 73
SP - 89
EP - 101
JO - Diseases of aquatic organisms
JF - Diseases of aquatic organisms
IS - 2
ER -