@inbook{114b00915c1b444eb77ccaeb628ef108,
title = "TYW1: A Radical SAM Enzyme Involved in the Biosynthesis of Wybutosine Bases",
abstract = "Transfer RNA is extensively modified by the actions of a variety of enzymes. The radical S-adenosyl-L-methionine enzyme TYW1 modifies tRNAPhe forming the characteristic tricyclic ring via the condensation of carbons 2 and 3 of pyruvate. This chapter details methods that are required for studies of TYW1.",
keywords = "Radical SAM, TYW1, Wyosine, tRNA, tRNA modification",
author = "Young, {Anthony P.} and Vahe Bandarian",
note = "Funding Information: This work was supported by the National Institutes of Health (GM072623 and GM120638). Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2018",
doi = "10.1016/bs.mie.2018.04.024",
language = "English (US)",
isbn = "9780128127940",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "119--153",
editor = "Vahe Bandarian",
booktitle = "Methods in Enzymology",
}