TY - JOUR
T1 - Two-Dimensional Strain Imaging of Controlled Rabbit Hearts
AU - Jia, Congxian
AU - Olafsson, Ragnar
AU - Kim, Kang
AU - Kolias, Theodore J.
AU - Rubin, Jonathan M.
AU - Weitzel, William F.
AU - Witte, Russell S.
AU - Huang, Sheng Wen
AU - Richards, Michael S.
AU - Deng, Cheri X.
AU - O'Donnell, Matthew
N1 - Funding Information:
The authors thank Nancy Roeser and Kimberly Ives for their generous help and training, Adam Lauver and Erin Booth for their help with the Langendorff setup and Marty Schlicht for lending the pressure transducer. The authors thank the support of NIH grants HL-082640, HL-67647, HL-68658, CA-109440 and EB-003451.
PY - 2009/9
Y1 - 2009/9
N2 - Ultrasound strain imaging using 2-D speckle tracking has been proposed to quantitatively assess changes in myocardial contractility caused by ischemia. Its performance must be demonstrated in a controlled model system as a step toward routine clinical application. In this study, a well-controlled 2-D cardiac elasticity imaging technique was developed using two coplanar and orthogonal linear probes simultaneously imaging an isolated retroperfused rabbit heart. Acute ischemia was generated by left anterior descending (LAD) artery ligation. An excitation-contraction decoupler, 2,3-butanedione monoxime, was applied at a 4-mM concentration to reversibly reduce myocardial contractility. Results using a single probe demonstrate that directional changes in the in-plane principal deformation axes can help locate the bulging area as a result of LAD ligation, which matched well with corresponding Evans Blue staining, and strains or strain magnitude, based on principal stretches, can characterize heart muscle contractility. These two findings using asymmetric displacement accuracy (i.e., normal single-probe measurements with good axial but poor lateral estimates) were further validated using symmetric displacement accuracy (i.e., dual-probe measurements using only accurate axial tracking estimates from each). However, the accuracy of 2-D cardiac strain imaging using a single probe depends on the probe's orientation because of the large variance in lateral displacement estimates. (E-mail: [email protected]).
AB - Ultrasound strain imaging using 2-D speckle tracking has been proposed to quantitatively assess changes in myocardial contractility caused by ischemia. Its performance must be demonstrated in a controlled model system as a step toward routine clinical application. In this study, a well-controlled 2-D cardiac elasticity imaging technique was developed using two coplanar and orthogonal linear probes simultaneously imaging an isolated retroperfused rabbit heart. Acute ischemia was generated by left anterior descending (LAD) artery ligation. An excitation-contraction decoupler, 2,3-butanedione monoxime, was applied at a 4-mM concentration to reversibly reduce myocardial contractility. Results using a single probe demonstrate that directional changes in the in-plane principal deformation axes can help locate the bulging area as a result of LAD ligation, which matched well with corresponding Evans Blue staining, and strains or strain magnitude, based on principal stretches, can characterize heart muscle contractility. These two findings using asymmetric displacement accuracy (i.e., normal single-probe measurements with good axial but poor lateral estimates) were further validated using symmetric displacement accuracy (i.e., dual-probe measurements using only accurate axial tracking estimates from each). However, the accuracy of 2-D cardiac strain imaging using a single probe depends on the probe's orientation because of the large variance in lateral displacement estimates. (E-mail: [email protected]).
KW - 2-D Speckle tracking
KW - Cardiac strain
KW - Langendorff
KW - Principal stretch
UR - http://www.scopus.com/inward/record.url?scp=68949099455&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68949099455&partnerID=8YFLogxK
U2 - 10.1016/j.ultrasmedbio.2009.04.007
DO - 10.1016/j.ultrasmedbio.2009.04.007
M3 - Article
C2 - 19616362
AN - SCOPUS:68949099455
SN - 0301-5629
VL - 35
SP - 1488
EP - 1501
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 9
ER -