@inbook{f737c0e02dcd466eb8c3f5ef9a0ca4ac,
title = "Translational control by helicases during cellular stress",
abstract = "Stress is inevitable, so all organisms have developed response mechanisms to allow for their survival during times of stress. Regulation of gene expression is a critical part of these responses, which allows for the appropriate cohort of proteins to be produced to counter the stress while downregulating others in order to conserve resources. Translation is both highly energy intensive and able to rapidly shift the proteome, thus making it a key target for regulation during stress. Numerous stress pathways converge on translation, and examining the regulatory mechanisms that underlie these pathways is essential for understanding the initial and long-term effects of stress on cells. A number of RNA helicases, including eIF4A, Ded1/DDX3X, and Dhh1/DDX6, have been previously linked to translation, and given their ability to dramatically alter RNA-protein interactions, they are well-positioned to play critical roles in translation regulation during stress. Therefore, assessing the role of helicases in these conditions is vital to the overall understanding of stress. Outlined below are key assays focusing on two areas: assessing cellular phenotypes in growth and survival during stress conditions, and analyzing cellular translation in the presence and absence of stress. The combination of these two approaches will begin to establish the function(s) of a given helicase in the overall stress response.",
keywords = "Growth curve, Helicase, Luciferase, Metabolic labeling, Polysome profile, Stress, Translation, Yeast",
author = "Carey, {Sara B.} and Bolger, {Timothy A.}",
note = "Funding Information: The authors would like to thank Michael Trakselis, Frederico Paulo Mendoza, and other editors at Methods in Enzymology for this opportunity. We would also like to thank current and former members of the Bolger lab for helpful advice and discussion. This work was partly supported by a grant from the National Institute of General Medical Sciences (R01-GM136827). Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",
year = "2022",
month = jan,
doi = "10.1016/bs.mie.2022.03.056",
language = "English (US)",
isbn = "9780323997737",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "103--140",
editor = "Trakselis, {Michael A.}",
booktitle = "Helicase Enzymes Part B",
}