TY - JOUR
T1 - Transgenesis by means of blastocyst-derived embryonic stem cell lines
AU - Gossler, A.
AU - Doetschman, T.
AU - Korn, R.
AU - Serfling, E.
AU - Kemler, R.
PY - 1986
Y1 - 1986
N2 - This study demonstrates that blastocyst-derived embryonic stem cells (ES cells) can be used as a vehicle for transgenesis. The method is nearly as efficient as other methods, and the introduced neomycin phosphotransferase (neo) gene is stably transmitted through several generations with no apparent loss in G418 resistance. An important factor contributing to the efficiency of this process is the rigorous selection, before blastocyst injection, of genetically transformed cells for in vitro developmental pluripotency. One of the advantages of the ES cell route to transgenesis is that it provides investigators with the opportunity to screen for the desired genetic alterations before reintroducing the ES cells into the animal.
AB - This study demonstrates that blastocyst-derived embryonic stem cells (ES cells) can be used as a vehicle for transgenesis. The method is nearly as efficient as other methods, and the introduced neomycin phosphotransferase (neo) gene is stably transmitted through several generations with no apparent loss in G418 resistance. An important factor contributing to the efficiency of this process is the rigorous selection, before blastocyst injection, of genetically transformed cells for in vitro developmental pluripotency. One of the advantages of the ES cell route to transgenesis is that it provides investigators with the opportunity to screen for the desired genetic alterations before reintroducing the ES cells into the animal.
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U2 - 10.1073/pnas.83.23.9065
DO - 10.1073/pnas.83.23.9065
M3 - Article
C2 - 3024164
AN - SCOPUS:0001256353
SN - 0027-8424
VL - 83
SP - 9065
EP - 9069
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 23
ER -