Transforming growth factor-β1 inhibits regeneration of renal proximal tubular cells after oxidant exposure

Sandra E. Kays, Grazyna Nowak, Rick G. Schnellmann

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Transforming growth factor-β (TGF-β), a regulatory cytokine expressed in the kidney, plays a role in nephrogenic repair. This study utilized a chemical model of renal proximal tubule cellular injury and regeneration to investigate the effects of TGF-β on regeneration. Confluent monolayers of rabbit renal proximal tubular cells (RPTC) in primary culture exposed to the oxidant t-butylhydroperoxide (800 μM TBHP) for 1.5 hours were 24% confluent after 24 hours. Confluency increased to 50% 4 days after TBHP exposure. Recovery of monolayer confluency was associated with increased monolayer protein but not with DNA content. Daily treatment of injured monolayers with TGF-β1 inhibited the recovery of monolayer confluency and inhibited recovery of protein content in a concentration-dependent manner (0.02-1 ng/mL). DNA content of injured monolayers was not altered by TGF-β1. A. single treatment of injured monolayers with 0.2 ng/mL (8 pM) TGF-β1 inhibited recovery of monolayer confluency and protein content without altering monolayer DNA content. These data show that a single 24 hour exposure to a low concentration (8 pM) of TGF-β1 inhibits regeneration of renal proximal tubule cell monolayers following oxidative injury by inhibiting, in part, cellular migration/spreading.

Original languageEnglish (US)
Pages (from-to)79-84
Number of pages6
JournalJournal of biochemical toxicology
Volume11
Issue number2
DOIs
StatePublished - 1996
Externally publishedYes

Keywords

  • Cellular injury and regeneration
  • Nephrogenic repair
  • Oxidative stress
  • Renal proximal tubular cells
  • t-Butylhydroperoxide
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Toxicology

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