TY - JOUR
T1 - Transforming growth factor-β1 inhibits regeneration of renal proximal tubular cells after oxidant exposure
AU - Kays, Sandra E.
AU - Nowak, Grazyna
AU - Schnellmann, Rick G.
PY - 1996
Y1 - 1996
N2 - Transforming growth factor-β (TGF-β), a regulatory cytokine expressed in the kidney, plays a role in nephrogenic repair. This study utilized a chemical model of renal proximal tubule cellular injury and regeneration to investigate the effects of TGF-β on regeneration. Confluent monolayers of rabbit renal proximal tubular cells (RPTC) in primary culture exposed to the oxidant t-butylhydroperoxide (800 μM TBHP) for 1.5 hours were 24% confluent after 24 hours. Confluency increased to 50% 4 days after TBHP exposure. Recovery of monolayer confluency was associated with increased monolayer protein but not with DNA content. Daily treatment of injured monolayers with TGF-β1 inhibited the recovery of monolayer confluency and inhibited recovery of protein content in a concentration-dependent manner (0.02-1 ng/mL). DNA content of injured monolayers was not altered by TGF-β1. A. single treatment of injured monolayers with 0.2 ng/mL (8 pM) TGF-β1 inhibited recovery of monolayer confluency and protein content without altering monolayer DNA content. These data show that a single 24 hour exposure to a low concentration (8 pM) of TGF-β1 inhibits regeneration of renal proximal tubule cell monolayers following oxidative injury by inhibiting, in part, cellular migration/spreading.
AB - Transforming growth factor-β (TGF-β), a regulatory cytokine expressed in the kidney, plays a role in nephrogenic repair. This study utilized a chemical model of renal proximal tubule cellular injury and regeneration to investigate the effects of TGF-β on regeneration. Confluent monolayers of rabbit renal proximal tubular cells (RPTC) in primary culture exposed to the oxidant t-butylhydroperoxide (800 μM TBHP) for 1.5 hours were 24% confluent after 24 hours. Confluency increased to 50% 4 days after TBHP exposure. Recovery of monolayer confluency was associated with increased monolayer protein but not with DNA content. Daily treatment of injured monolayers with TGF-β1 inhibited the recovery of monolayer confluency and inhibited recovery of protein content in a concentration-dependent manner (0.02-1 ng/mL). DNA content of injured monolayers was not altered by TGF-β1. A. single treatment of injured monolayers with 0.2 ng/mL (8 pM) TGF-β1 inhibited recovery of monolayer confluency and protein content without altering monolayer DNA content. These data show that a single 24 hour exposure to a low concentration (8 pM) of TGF-β1 inhibits regeneration of renal proximal tubule cell monolayers following oxidative injury by inhibiting, in part, cellular migration/spreading.
KW - Cellular injury and regeneration
KW - Nephrogenic repair
KW - Oxidative stress
KW - Renal proximal tubular cells
KW - t-Butylhydroperoxide
KW - Transforming growth factor-β
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U2 - 10.1002/(SICI)1522-7146(1996)11:2<79::AID-JBT5>3.0.CO;2-R
DO - 10.1002/(SICI)1522-7146(1996)11:2<79::AID-JBT5>3.0.CO;2-R
M3 - Article
C2 - 8884468
AN - SCOPUS:0030331938
SN - 0887-2082
VL - 11
SP - 79
EP - 84
JO - Journal of biochemical toxicology
JF - Journal of biochemical toxicology
IS - 2
ER -