Transcriptional Control of Activation-Induced Cytidine Deaminase and Error-Prone DNA Polymerases Is Functionally Mature in the B Cells of Infants at Birth

Amber L. Bowen, Cuixia Tian, Bonnie J. LaFleur, James E. Crowe

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Somatic hypermutation (SHM) of immunoglobulin genes requires activation-induced cytidine deaminase (AID). The error-prone DNA polymerases, such as Pol η, Pol ζ, and Pol ι, also have been implicated in the process. Human adult antibodies directed to microbial pathogens are increased in affinity and function compared with those of infants. Adult antibodies achieve this increased affinity through somatic mutations, which are lacking in the B cells of infants. It is unknown if infant B cells are capable of upregulating the cell machinery needed to introduce mutations after stimulation through the antigen receptor. We show here that infant B cells exhibit similar kinetics and magnitude of transcription of AID and pol η genes and only marginally lower levels of pol ι and pol ζ genes after stimulation through the B cell receptor. These data suggest that the ability to upregulate gene transcription of enzymes mediating SHM is not a limiting determinant of the functional quality of infant antibody responses.

Original languageEnglish (US)
Pages (from-to)43-46
Number of pages4
JournalHuman Immunology
Volume67
Issue number1-2
DOIs
StatePublished - Jan 2006
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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