Toxicity and biotransformation of volatile halogenated anesthetics in rat hepatocyte suspensions

A. B. Direnzo, A. J. Gandolfi, S. D. Brooks, K. Brendel

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


This study, examines the toxicity (as measured by reduced intracellular K+ and metabolism (defluorination) of halothane and enflurane in rat hepatocytes in suspension (RHS) with regards to O2 tension, time, and concentration. In 95% O2 halothane is more toxic than enflurane when RHS are exposed to 5-20 μ1 of these anesthetics. At these levels halothane is not metabolized while enflurane is metabolized. At 21% O2 a similar pattern was seen with regards to toxicity. However, metabolism of halothane rapidly reached an elevated level while that of enflurane is reduced when compared to 95% O2. Thus toxicity of halothane and enflurane at these dose levels appears to be unrelated to metabolism and due solely to a solvent effect.

Original languageEnglish (US)
Pages (from-to)207-218
Number of pages12
JournalDrug and Chemical Toxicology
Issue number4
StatePublished - 1985

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety


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