Toluene-nitrite inhibition synergy of anaerobic ammonium oxidizing (anammox) activity

Sergio Martínez Hernández; Wenjie Sun; Reyes Sierra-Alvarez; Jim A. Field

doi:10.1016/j.procbio.2013.04.010

Toluene-nitrite inhibition synergy of anaerobic ammonium oxidizing (anammox) activity

Sergio Martínez Hernández, Wenjie Sun, Reyes Sierra-Alvarez, Jim A. Field

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Anammox process is emerging as a promising biotechnology to remove ammonium (NH₄⁺) from wastewater. Co-existing organic matters such as toluene could pose potential inhibitions on this process. In this study, the mechanisms of toluene-nitrite (NO₂^-) inhibition synergy of anammox activity (AA) was investigated in batch culture. In results, the AA (mmolN₂-NLliquid-1h^-1) in reference assays achieved values of 0.21 ± 0.0078. The toluene (0.1-3.0 mM) demonstrated different inhibitory effects on the AA and caused 50% inhibition (IC₅₀) at 0.79 mM. The pre-exposure to toluene and NO₂^- or NO ₂^- alone in the absence of NH₄⁺ demonstrated a much severer inhibition, compared to toluene alone. The IC ₅₀ of the AA due to pre-exposure to NO₂^- lacking NH₄⁺ was of 6.25 mM NO₂^-. These results indicated that toluene by itself did not severely inhibit the anammox process but did so in the presence of NO₂^-.

Original language	English (US)
Pages (from-to)	926-930
Number of pages	5
Journal	Process Biochemistry
Volume	48
Issue number	5-6
DOIs	https://doi.org/10.1016/j.procbio.2013.04.010
State	Published - May 2013

Keywords

Anammox
Anammox activity
Batch assay
Inhibition synergy
Nitrite
Toluene

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2013.04.010

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title = "Toluene-nitrite inhibition synergy of anaerobic ammonium oxidizing (anammox) activity",

abstract = "Anammox process is emerging as a promising biotechnology to remove ammonium (NH4+) from wastewater. Co-existing organic matters such as toluene could pose potential inhibitions on this process. In this study, the mechanisms of toluene-nitrite (NO2-) inhibition synergy of anammox activity (AA) was investigated in batch culture. In results, the AA (mmolN2-NLliquid-1h-1) in reference assays achieved values of 0.21 ± 0.0078. The toluene (0.1-3.0 mM) demonstrated different inhibitory effects on the AA and caused 50% inhibition (IC50) at 0.79 mM. The pre-exposure to toluene and NO2- or NO 2- alone in the absence of NH4+ demonstrated a much severer inhibition, compared to toluene alone. The IC 50 of the AA due to pre-exposure to NO2- lacking NH4+ was of 6.25 mM NO2-. These results indicated that toluene by itself did not severely inhibit the anammox process but did so in the presence of NO2-.",

keywords = "Anammox, Anammox activity, Batch assay, Inhibition synergy, Nitrite, Toluene",

author = "Hern{\'a}ndez, {Sergio Mart{\'i}nez} and Wenjie Sun and Reyes Sierra-Alvarez and Field, {Jim A.}",

note = "Funding Information: Participation of Sergio Mart{\'i}nez Hern{\'a}ndez as postdoctoral researcher was funded by the Council of Science and Technology of Mexico (CONACYT, grant no. 119093 ). Funding Information: The work presented here was funded by the University of Arizona , Water Sustainability Program. The anammox granular sludge samples were kindly provided by Paques BV, The Netherlands. The use of trade, product, or firm names in this report is for descriptive purposes only. ",

year = "2013",

month = may,

doi = "10.1016/j.procbio.2013.04.010",

language = "English (US)",

volume = "48",

pages = "926--930",

journal = "Process Biochemistry",

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T1 - Toluene-nitrite inhibition synergy of anaerobic ammonium oxidizing (anammox) activity

AU - Hernández, Sergio Martínez

AU - Sun, Wenjie

AU - Sierra-Alvarez, Reyes

AU - Field, Jim A.

N1 - Funding Information: Participation of Sergio Martínez Hernández as postdoctoral researcher was funded by the Council of Science and Technology of Mexico (CONACYT, grant no. 119093 ). Funding Information: The work presented here was funded by the University of Arizona , Water Sustainability Program. The anammox granular sludge samples were kindly provided by Paques BV, The Netherlands. The use of trade, product, or firm names in this report is for descriptive purposes only.

PY - 2013/5

Y1 - 2013/5

N2 - Anammox process is emerging as a promising biotechnology to remove ammonium (NH4+) from wastewater. Co-existing organic matters such as toluene could pose potential inhibitions on this process. In this study, the mechanisms of toluene-nitrite (NO2-) inhibition synergy of anammox activity (AA) was investigated in batch culture. In results, the AA (mmolN2-NLliquid-1h-1) in reference assays achieved values of 0.21 ± 0.0078. The toluene (0.1-3.0 mM) demonstrated different inhibitory effects on the AA and caused 50% inhibition (IC50) at 0.79 mM. The pre-exposure to toluene and NO2- or NO 2- alone in the absence of NH4+ demonstrated a much severer inhibition, compared to toluene alone. The IC 50 of the AA due to pre-exposure to NO2- lacking NH4+ was of 6.25 mM NO2-. These results indicated that toluene by itself did not severely inhibit the anammox process but did so in the presence of NO2-.

AB - Anammox process is emerging as a promising biotechnology to remove ammonium (NH4+) from wastewater. Co-existing organic matters such as toluene could pose potential inhibitions on this process. In this study, the mechanisms of toluene-nitrite (NO2-) inhibition synergy of anammox activity (AA) was investigated in batch culture. In results, the AA (mmolN2-NLliquid-1h-1) in reference assays achieved values of 0.21 ± 0.0078. The toluene (0.1-3.0 mM) demonstrated different inhibitory effects on the AA and caused 50% inhibition (IC50) at 0.79 mM. The pre-exposure to toluene and NO2- or NO 2- alone in the absence of NH4+ demonstrated a much severer inhibition, compared to toluene alone. The IC 50 of the AA due to pre-exposure to NO2- lacking NH4+ was of 6.25 mM NO2-. These results indicated that toluene by itself did not severely inhibit the anammox process but did so in the presence of NO2-.

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KW - Inhibition synergy

KW - Nitrite

KW - Toluene

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Toluene-nitrite inhibition synergy of anaerobic ammonium oxidizing (anammox) activity

Abstract

Keywords

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