Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide: Design, Synthesis, and Application of a Lanthanide-Based Probe

Yao Yao; Loruhama Delgado-Rivera; Hamid Samareh Afsari; Liang Yin; Gregory R.J. Thatcher; Terry W. Moore; Lawrence W. Miller

doi:10.1021/acs.inorgchem.7b02533

Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide: Design, Synthesis, and Application of a Lanthanide-Based Probe

Yao Yao
, Loruhama Delgado-Rivera
, Hamid Samareh Afsari
, Liang Yin
, Gregory R.J. Thatcher
, Terry W. Moore
, Lawrence W. Miller

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Hydrogen sulfide (H₂S) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for H₂S, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control millisecond-scale-lifetime-Tb(III)- or Eu(III)-emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively, and with high sensitivity to aqueous H₂S. The probe's potential for biological applications was verified by measuring the H₂S generated by the slow-releasing chemical-sulfide-donor GYY4147, by cystathionine γ-lyase (CSE), and by Na₂S-stimulated HeLa cells.

Original language	English (US)
Pages (from-to)	681-688
Number of pages	8
Journal	Inorganic Chemistry
Volume	57
Issue number	2
DOIs	https://doi.org/10.1021/acs.inorgchem.7b02533
State	Published - Jan 16 2018
Externally published	Yes

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Inorganic Chemistry

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10.1021/acs.inorgchem.7b02533

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title = "Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide: Design, Synthesis, and Application of a Lanthanide-Based Probe",

abstract = "Hydrogen sulfide (H2S) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for H2S, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control millisecond-scale-lifetime-Tb(III)- or Eu(III)-emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively, and with high sensitivity to aqueous H2S. The probe's potential for biological applications was verified by measuring the H2S generated by the slow-releasing chemical-sulfide-donor GYY4147, by cystathionine γ-lyase (CSE), and by Na2S-stimulated HeLa cells.",

author = "Yao Yao and Loruhama Delgado-Rivera and \{Samareh Afsari\}, Hamid and Liang Yin and Thatcher, \{Gregory R.J.\} and Moore, \{Terry W.\} and Miller, \{Lawrence W.\}",

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year = "2018",

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doi = "10.1021/acs.inorgchem.7b02533",

language = "English (US)",

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pages = "681--688",

journal = "Inorganic Chemistry",

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TY - JOUR

T1 - Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide

T2 - Design, Synthesis, and Application of a Lanthanide-Based Probe

AU - Yao, Yao

AU - Delgado-Rivera, Loruhama

AU - Samareh Afsari, Hamid

AU - Yin, Liang

AU - Thatcher, Gregory R.J.

AU - Moore, Terry W.

AU - Miller, Lawrence W.

PY - 2018/1/16

Y1 - 2018/1/16

N2 - Hydrogen sulfide (H2S) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for H2S, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control millisecond-scale-lifetime-Tb(III)- or Eu(III)-emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively, and with high sensitivity to aqueous H2S. The probe's potential for biological applications was verified by measuring the H2S generated by the slow-releasing chemical-sulfide-donor GYY4147, by cystathionine γ-lyase (CSE), and by Na2S-stimulated HeLa cells.

AB - Hydrogen sulfide (H2S) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for H2S, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control millisecond-scale-lifetime-Tb(III)- or Eu(III)-emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively, and with high sensitivity to aqueous H2S. The probe's potential for biological applications was verified by measuring the H2S generated by the slow-releasing chemical-sulfide-donor GYY4147, by cystathionine γ-lyase (CSE), and by Na2S-stimulated HeLa cells.

UR - https://www.scopus.com/pages/publications/85040820261

UR - https://www.scopus.com/pages/publications/85040820261#tab=citedBy

U2 - 10.1021/acs.inorgchem.7b02533

DO - 10.1021/acs.inorgchem.7b02533

M3 - Article

C2 - 29281273

AN - SCOPUS:85040820261

SN - 0020-1669

VL - 57

SP - 681

EP - 688

JO - Inorganic Chemistry

JF - Inorganic Chemistry

IS - 2

ER -

Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide: Design, Synthesis, and Application of a Lanthanide-Based Probe

Abstract

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