Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide: Design, Synthesis, and Application of a Lanthanide-Based Probe

Yao Yao, Loruhama Delgado-Rivera, Hamid Samareh Afsari, Liang Yin, Gregory R.J. Thatcher, Terry W. Moore, Lawrence W. Miller

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Hydrogen sulfide (H2S) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for H2S, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control millisecond-scale-lifetime-Tb(III)- or Eu(III)-emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively, and with high sensitivity to aqueous H2S. The probe's potential for biological applications was verified by measuring the H2S generated by the slow-releasing chemical-sulfide-donor GYY4147, by cystathionine γ-lyase (CSE), and by Na2S-stimulated HeLa cells.

Original languageEnglish (US)
Pages (from-to)681-688
Number of pages8
JournalInorganic Chemistry
Volume57
Issue number2
DOIs
StatePublished - Jan 16 2018
Externally publishedYes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Inorganic Chemistry

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