TY - JOUR
T1 - Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide
T2 - Design, Synthesis, and Application of a Lanthanide-Based Probe
AU - Yao, Yao
AU - Delgado-Rivera, Loruhama
AU - Samareh Afsari, Hamid
AU - Yin, Liang
AU - Thatcher, Gregory R.J.
AU - Moore, Terry W.
AU - Miller, Lawrence W.
N1 - Funding Information:
We are grateful to the National Institutes of Health (NHLBI UH2 L123610 and NIGMS R01 GM081030) and the UICentre for Drug Discovery, University of Illinois at Chicago, Office of the Vice Chancellor for Research.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2018/1/16
Y1 - 2018/1/16
N2 - Hydrogen sulfide (H2S) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for H2S, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control millisecond-scale-lifetime-Tb(III)- or Eu(III)-emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively, and with high sensitivity to aqueous H2S. The probe's potential for biological applications was verified by measuring the H2S generated by the slow-releasing chemical-sulfide-donor GYY4147, by cystathionine γ-lyase (CSE), and by Na2S-stimulated HeLa cells.
AB - Hydrogen sulfide (H2S) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for H2S, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control millisecond-scale-lifetime-Tb(III)- or Eu(III)-emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively, and with high sensitivity to aqueous H2S. The probe's potential for biological applications was verified by measuring the H2S generated by the slow-releasing chemical-sulfide-donor GYY4147, by cystathionine γ-lyase (CSE), and by Na2S-stimulated HeLa cells.
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U2 - 10.1021/acs.inorgchem.7b02533
DO - 10.1021/acs.inorgchem.7b02533
M3 - Article
C2 - 29281273
AN - SCOPUS:85040820261
SN - 0020-1669
VL - 57
SP - 681
EP - 688
JO - Inorganic Chemistry
JF - Inorganic Chemistry
IS - 2
ER -