Time course of ICAM-1 expression and leukocyte subset infiltration in rat forebrain ischemia

Wayne M. Clark, Jeffrey D. Lauten, Nikola Lessov, William Woodward, Bruce M. Coull

Research output: Contribution to journalArticlepeer-review

70 Scopus citations


The time course of ICAM-1 expression and leukocyte subset infiltration was studied in a model of CNS reperfusion injury in adult rats. Leukocyte adhesion and infiltration, mediated in part by intercellular adhesion molecule-1 (ICAM-1), appears to potentiate CNS reperfusion injury. The timing and relationship between ICAM-1 staining and leukocyte infiltration postglobal CNS ischemia is unknown. Reversible forebrain ischemia was produced in 32 adult Sprague-Dawley rats using the two-vessel occlusion model with histologic analysis performed at specific intervals postischemia: 1, 3, 6, 12 and 24h, 4 and 7 d, or sham-operated controls (n=4 each group). Monoclonal antibodies against ICAM-1 (1A29 and TM8), a specific granulocyte (PMN) (HIS48), and a specific monocyte/macrophage (MØ)(ED1) were used. No specific leukocyte and only rare ICAM-1 vessel immunoreactivity was observed in sham controls. ICAM-1: Significant expression in microvessels beginning at 1 h with additional diffuse CA1 pyramidal layer staining beginning at 4 d. Leukocytes: NO PMN cells and rare MØ identified at 6 and 12 h. By 24 h: moderate infiltrate in areas of ICAM-1 expression of PMN and MØ. At 4 and 7 d: only MØ accumulation, cellular morphology now similar to microglia. The results of this study indicate that early and persistent ICAM-1 expression occurs following CNS ischemia with associated leukocyte infiltration.

Original languageEnglish (US)
Pages (from-to)213-230
Number of pages18
JournalMolecular and Chemical Neuropathology
Issue number3
StatePublished - Dec 1995


  • ICAM-1
  • cerebral ischemia
  • leukocytes
  • macrophage
  • microglia
  • reperfusion injury

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology


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