Three-Dimensional Segmentation of the Ex-Vivo Anterior Lamina Cribrosa from Second-Harmonic Imaging Microscopy

Sundaresh Ram, Forest Danford, Stephen Howerton, Jeffrey J. Rodríguez, Jonathan P.Vande Geest

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The lamina cribrosa (LC) is a connective tissue in the posterior eye with a complex mesh-like trabecular microstructure, through which all the retinal ganglion cell axons and central retinal vessels pass. Recent studies have demonstrated that changes in the structure of the LC correlate with glaucomatous damage. Thus, accurate segmentation and reconstruction of the LC is of utmost importance. This paper presents a new automated method for segmenting the microstructure of the anterior LC in the images obtained via multiphoton microscopy using a combination of ideas. In order to reduce noise, we first smooth the input image using a 4-D collaborative filtering scheme. Next, we enhance the beam-like trabecular microstructure of the LC using wavelet multiresolution analysis. The enhanced LC microstructure is then automatically extracted using a combination of histogram thresholding and graph-cut binarization. Finally, we use morphological area opening as a postprocessing step to remove the small and unconnected 3-D regions in the binarized images. The performance of the proposed method is evaluated using mutual overlap accuracy, Tanimoto index, F-score, and Rand index. Quantitative and qualitative results show that the proposed algorithm provides improved segmentation accuracy and computational efficiency compared to the other recent algorithms.

Original languageEnglish (US)
Pages (from-to)1617-1629
Number of pages13
JournalIEEE Transactions on Biomedical Engineering
Issue number7
StatePublished - Jul 2018


  • Graph cut segmentation
  • histogram thresholding
  • lamina cribrosa
  • volumetric data denoising
  • wavelet

ASJC Scopus subject areas

  • Biomedical Engineering


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