Abstract
Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (~1-3 cm3) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.
Original language | English (US) |
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Pages (from-to) | 2664-2679 |
Number of pages | 16 |
Journal | Biomaterials |
Volume | 35 |
Issue number | 9 |
DOIs | |
State | Published - Mar 2014 |
Keywords
- Acellular matrix
- Endothelial cell
- Epithelial cell
- Extracellular matrix (ECM)
- Human lung fibroblast
- Mesenchymal stem cell
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Ceramics and Composites
- Biomaterials
- Mechanics of Materials