@inbook{c2ea42a31f7543ae98bf48e1e8e66d96,
title = "Three-Dimensional Reconstruction of Toxoplasma–Neuron Interactions In Situ",
abstract = "How tissue and cellular architecture affects host cell–microbe interactions in vivo remains poorly defined because imaging these interactions in complex tissue is difficult and standard in vitro cultures do not mimic whole organ architecture. Here we describe a method that combines new tissue clearing techniques, high-resolution imaging, and three-dimensional reconstruction to overcome these barriers and allow in situ imaging of host cell–microbe interactions in complex tissue. We use the interactions between neurons and Toxoplasma gondii, a ubiquitous, protozoan parasite that establish a lifelong central nervous system (CNS) infection in mice and humans, as a model for this technique. This method aims to provide an easy, reproducible way to visualize the complex relationship between host cells and intracellular pathogens within a whole organ.",
keywords = "3-D reconstruction, Confocal microscopy, Host-microbe interactions, Tissue clearing, Toxoplasma",
author = "Cabral, {Carla M.} and Merritt, {Emily F.} and Koshy, {Anita A.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-4939-9857-9_16",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "283--295",
booktitle = "Methods in Molecular Biology",
}