Three-dimensional reconstruction of brain capillaries from frozen serial sections

D. Farquhar, T. L. Loo, J. U. Gutterman, Evan M Hersh, M. A. Luna

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Based on frozen serial sections and microphotographs, the present study describes a three-dimensional reconstruction of the alkaline-phosphatase positive capillary network in the cat's cerebral cortex. This model clearly demonstrates a continuous increase of the volumetric capillary density towards the deeper cortical layers. Results of a morphometric investigation subsequently performed on photographed capillaries and obtained from a relatively small tissue sample fit well with the data from the whole temporo-parietal cortex region. The correspondence between the two data sets reflects specific morphometric characteristics of the capillary net in the cortex of the temporo-parietal Suprasylvian Gyrus. The information obtained from the three-dimensional capillary model represents a basic tool for additional computer-assisted steric reconstruction of the capillary network in the cerebral cortex.

Original languageEnglish (US)
Pages (from-to)175-180
Number of pages6
JournalMicrovascular Research
Issue number2
StatePublished - Mar 1976

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology


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