Thimerosal modulates the agoplist-specific cytosolic Ca2+ oscillatory patterns in single pancreatic acinar cells of mouse

Jie Wu, Teruko Takeo, Noritaka Kamimura, Junro Wada, Sechiko Suga, Yuko Hoshina, Makoto Wakui

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Modulation of the agonist-specific cytosolic Ca2+ oscillatory pattern by thimerosal has been investigated in single pancreatic acinar cells using patch-clamp perforated whole-cell recording to measure the calcium-dependent chloride current (I(C1(Ca2+)). 1 μM thimerosal, which fails to evoke Ca2+ oscillation alone, clearly changed the pattern of Ca2+ oscillation from pulsatile spikes (evoked by low concentrations of activators) to sinusoidal or transient oscillations. The mimetic action of thimerosal was independent of extracellular Ca2+, was blocked by extracellular application of dithiothreitol or 10 mM caffeine, as well as by internal perfusion with heparin; but was unaffected by ruthenium red. We conclude that thimerosal modulates the agonist-specific cytosolic Ca2+ oscillatory patterns mediated by sensitizing the InsP3-induced Ca2+ release.

Original languageEnglish (US)
Pages (from-to)149-152
Number of pages4
JournalFEBS Letters
Volume390
Issue number2
DOIs
StatePublished - Jul 22 1996
Externally publishedYes

Keywords

  • Ca oscillation
  • Ca-dependent Cl current
  • Inositol trisphosphate
  • Pancreatic acinar cell
  • Patch-clamp
  • Thimerosal

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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