Thermodynamics of Glucagon Aggregation

Robert E. Johnson; Victor J. Hruby; John A. Rupley

doi:10.1021/bi00574a009

Thermodynamics of Glucagon Aggregation

Robert E. Johnson, Victor J. Hruby, John A. Rupley

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16 Scopus citations

Abstract

Heats of dilution of concentrated glucagon solutions have been measured calorimetrically at 10 and 25 °C in 0.2 M potassium phosphate buffer of pH 10.6. Analysis of the data in terms of a monomer-trimer equilibrium gives the following thermodynamic parameters for the association reaction at 25 °C: ΔG°=-7.34 kcal/mol of trimer, ΔH°=-31.2 kcal/mol, ΔS°=-80 cal/(K mol), ΔCp=-430 cal/(K mol). The sensitivity of heat of dilution data to the association constant and stoichiometry of the reaction is discussed.

Original language	English (US)
Pages (from-to)	1176-1179
Number of pages	4
Journal	Biochemistry
Volume	18
Issue number	7
DOIs	https://doi.org/10.1021/bi00574a009
State	Published - 1979
Externally published	Yes

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Biochemistry

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title = "Thermodynamics of Glucagon Aggregation",

abstract = "Heats of dilution of concentrated glucagon solutions have been measured calorimetrically at 10 and 25 °C in 0.2 M potassium phosphate buffer of pH 10.6. Analysis of the data in terms of a monomer-trimer equilibrium gives the following thermodynamic parameters for the association reaction at 25 °C: ΔG°=-7.34 kcal/mol of trimer, ΔH°=-31.2 kcal/mol, ΔS°=-80 cal/(K mol), ΔCp=-430 cal/(K mol). The sensitivity of heat of dilution data to the association constant and stoichiometry of the reaction is discussed.",

author = "Johnson, {Robert E.} and Hruby, {Victor J.} and Rupley, {John A.}",

year = "1979",

doi = "10.1021/bi00574a009",

language = "English (US)",

volume = "18",

pages = "1176--1179",

journal = "Biochemistry",

issn = "0006-2960",

publisher = "American Chemical Society",

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T1 - Thermodynamics of Glucagon Aggregation

AU - Johnson, Robert E.

AU - Hruby, Victor J.

AU - Rupley, John A.

PY - 1979

Y1 - 1979

N2 - Heats of dilution of concentrated glucagon solutions have been measured calorimetrically at 10 and 25 °C in 0.2 M potassium phosphate buffer of pH 10.6. Analysis of the data in terms of a monomer-trimer equilibrium gives the following thermodynamic parameters for the association reaction at 25 °C: ΔG°=-7.34 kcal/mol of trimer, ΔH°=-31.2 kcal/mol, ΔS°=-80 cal/(K mol), ΔCp=-430 cal/(K mol). The sensitivity of heat of dilution data to the association constant and stoichiometry of the reaction is discussed.

AB - Heats of dilution of concentrated glucagon solutions have been measured calorimetrically at 10 and 25 °C in 0.2 M potassium phosphate buffer of pH 10.6. Analysis of the data in terms of a monomer-trimer equilibrium gives the following thermodynamic parameters for the association reaction at 25 °C: ΔG°=-7.34 kcal/mol of trimer, ΔH°=-31.2 kcal/mol, ΔS°=-80 cal/(K mol), ΔCp=-430 cal/(K mol). The sensitivity of heat of dilution data to the association constant and stoichiometry of the reaction is discussed.

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DO - 10.1021/bi00574a009

M3 - Article

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SN - 0006-2960

VL - 18

SP - 1176

EP - 1179

JO - Biochemistry

JF - Biochemistry

IS - 7

ER -

Thermodynamics of Glucagon Aggregation

Abstract

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