TY - JOUR
T1 - The utility of immunohistochemical detection of DNA mismatch repair gene proteins
AU - Shia, Jinru
AU - Ellis, Nathan A.
AU - Klimstra, David S.
PY - 2004/11
Y1 - 2004/11
N2 - Since the development of monoclonal antibodies against the MSH2 protein by Leach et al. in 1996, a series of investigations has been undertaken to determine the utility of immunohistochemical detection of DNA mismatch repair (MMR) gene proteins in the identification of hereditary or sporadic colorectal tumors with microsatellite instability. These studies, however, have been performed with different aims and on different patient populations. Interpretation of these immunohistochemical data relies on a thorough understanding of the biological and technical factors that affect the detection of MMR proteins. In this review, we analyze the data from the published research studies, pointing out the various factors affecting immunohistochemical detection of MMR proteins and projecting the utility of immunohistochemistry in different clinical settings.
AB - Since the development of monoclonal antibodies against the MSH2 protein by Leach et al. in 1996, a series of investigations has been undertaken to determine the utility of immunohistochemical detection of DNA mismatch repair (MMR) gene proteins in the identification of hereditary or sporadic colorectal tumors with microsatellite instability. These studies, however, have been performed with different aims and on different patient populations. Interpretation of these immunohistochemical data relies on a thorough understanding of the biological and technical factors that affect the detection of MMR proteins. In this review, we analyze the data from the published research studies, pointing out the various factors affecting immunohistochemical detection of MMR proteins and projecting the utility of immunohistochemistry in different clinical settings.
KW - DNA mismatch repair gene proteins
KW - Immunohistochemistry
KW - Microsatellite instability
UR - http://www.scopus.com/inward/record.url?scp=9944243710&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9944243710&partnerID=8YFLogxK
U2 - 10.1007/s00428-004-1090-5
DO - 10.1007/s00428-004-1090-5
M3 - Review article
C2 - 15455227
AN - SCOPUS:9944243710
SN - 0945-6317
VL - 445
SP - 431
EP - 441
JO - Virchows Archiv - Abteilung A Pathologische Anatomie
JF - Virchows Archiv - Abteilung A Pathologische Anatomie
IS - 5
ER -