TY - JOUR
T1 - The use of wheat germ agglutinin-horseradish peroxidase conjugates for studies of anterograde axonal transport
AU - Mantyh, Patrick W.
AU - Peschanski, Marc
PY - 1983/2
Y1 - 1983/2
N2 - Injection of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) into the hemisected spinal cord of the rat, cat and monkey consistently resulted in the intense anterograde labeling of ascending spinal projections such as the spinothalamic tract and spinocerebellar tracts and their terminal fields. Injections of WGA-HRP in the dorsal column nuclei resulted in the anterograde labeling of the medial lemniscus and its terminal fields in the thalamus. Injection of similar amounts of horseradish peroxidase alone (HRP) in hemisected animals or the dorsal column nuclei resulted in little anterograde labeling. The rate of the anterograde transport of WGA-HRP in cut axons appears to be greater than 200 mm/day. Small amounts of transneuronal labeling appeared to occur after injection of WGA-HRP in both cut axons and undamaged cell bodies. These results suggest that the amount of anterograde labeling observed after injection of WGA-HRP into both cut axons and undamaged cell bodies is significantly greater than the anterograde labeling observed after injections of HRP alone. Therefore, in the central nervous system WGA-HRP appears to be a far more effective anterograde tracer than HRP alone.
AB - Injection of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) into the hemisected spinal cord of the rat, cat and monkey consistently resulted in the intense anterograde labeling of ascending spinal projections such as the spinothalamic tract and spinocerebellar tracts and their terminal fields. Injections of WGA-HRP in the dorsal column nuclei resulted in the anterograde labeling of the medial lemniscus and its terminal fields in the thalamus. Injection of similar amounts of horseradish peroxidase alone (HRP) in hemisected animals or the dorsal column nuclei resulted in little anterograde labeling. The rate of the anterograde transport of WGA-HRP in cut axons appears to be greater than 200 mm/day. Small amounts of transneuronal labeling appeared to occur after injection of WGA-HRP in both cut axons and undamaged cell bodies. These results suggest that the amount of anterograde labeling observed after injection of WGA-HRP into both cut axons and undamaged cell bodies is significantly greater than the anterograde labeling observed after injections of HRP alone. Therefore, in the central nervous system WGA-HRP appears to be a far more effective anterograde tracer than HRP alone.
KW - anterograde transport
KW - dorsal column nuclei
KW - spinothalamic tract
KW - transneuronal transport
KW - wheat germ agglutinin-HRP
UR - http://www.scopus.com/inward/record.url?scp=0020604133&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020604133&partnerID=8YFLogxK
U2 - 10.1016/0165-0270(83)90074-2
DO - 10.1016/0165-0270(83)90074-2
M3 - Article
C2 - 6188004
AN - SCOPUS:0020604133
SN - 0165-0270
VL - 7
SP - 117
EP - 128
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 2
ER -