@inbook{f710163dc94f4a568638aebba681028b,
title = "The Use of Flow Cytometry for Estimating Genome Sizes and DNA Ploidy Levels in Plants",
abstract = "Flow cytometry has emerged as a uniquely flexible, accurate, and widely applicable technology for the analysis of plant cells. One of its most important applications centers on the measurement of nuclear DNA contents. This chapter describes the essential features of this measurement, outlining the overall methods and strategies, but going on to provide a wealth of technical details to ensure the most accurate and reproducible results. The chapter is aimed to be equally accessible to experienced plant cytometrists as well as those newly entering the field. Besides providing a step-by-step guide for estimating genome sizes and DNA-ploidy levels from fresh tissues, special attention is paid to the use of seeds and desiccated tissues for such purposes. Methodological aspects regarding field sampling, transport, and storage of plant material are also given in detail. Finally, troubleshooting information for the most common problems that may arise during the application of these methods is provided.",
keywords = "Best practices, DAPI, Desiccated tissues, DNA-ploidy level, Flow cytometry, Genome size, Plant nuclei isolation, Plant tissues, Propidium iodide, Seeds",
author = "Jo{\~a}o Loureiro and Martin {\v C}ertner and Magdalena Lu{\v c}anov{\'a} and Elwira Sliwinska and Filip Kol{\'a}{\v r} and Jaroslav Dole{\v z}el and S{\`o}nia Garcia and S{\'i}lvia Castro and Galbraith, {David W.}",
note = "Funding Information: We thank Petr C{\'a}pal for drawing Fig. 1. J.L. was supported by Project RENATURE financed by the “Programa Operacional Regional do Centro 2014-2020 (Centro 2020)-CENTRO-01-0145-FEDER-000007{"}, and by CFE (FCT/UIDB/04004/2020). S.C. was financed by CULTIVAR project (CENTRO-01-0145-FEDER-000020), cofinanced by the Regional Operational Programme Centro 2020, Portugal 2020 and European Union, through European Fund for Regional Development (ERDF). J.D. was supported from ERDF project “Plants as a tool for sustainable global development” (No. CZ.02.1.01/0.0/0.0/16_019/0000827). M.{\v C}., M.L., and F.K. were supported from the long-term Research Development Project RVO67985939 (Czech Academy of Sciences). Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3226-0_2",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "25--64",
booktitle = "Methods in Molecular Biology",
}