The use of an infectious hypodermal and hematopoietic necrosis virus gene probe serodiagnostic field kit for the screening of candidate specific pathogen-free Penaeus vannamei broodstock

An experimental non-lethal serodiagnostic kit was evaluated for screening candidate specific pathogen-free (SPF) Penaeus vannamei broodstock under typical field conditions. In this trial, a total of 26 adult P. vannamei was screened for the presence of infectious hypodermal and hematopoietic necrosis virus (IHHNV) using an IHHNV dot blot field kit. The non-radioactive IHHNV in situ hybridization method was used as a standard for comparison. The results showed a significant relationship between the outcome of the dot blot kit and the outcome of the in situ method. In particular, the test results using the experimental kit were consistent with the in situ hybridization method. Based on these findings, the experimental serodiagnostic kit may provide a more rapid, cost-effective diagnostic method than traditional techniques for IHHNV detection.