The S100P/RAGE signaling pathway regulates expression of microRNA-21 in colon cancer cells

Melania E. Mercado-Pimentel; Benjamin C. Onyeagucha; Qing Li; Angel C. Pimentel; Jana Jandova; Mark A. Nelson

doi:10.1016/j.febslet.2015.07.010

The S100P/RAGE signaling pathway regulates expression of microRNA-21 in colon cancer cells

Melania E. Mercado-Pimentel, Benjamin C. Onyeagucha, Qing Li, Angel C. Pimentel, Jana Jandova, Mark A. Nelson

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Abstract S100P signaling through the receptor for advanced glycation end-products (RAGE) contributes to colon cancer invasion and metastasis, but the mechanistic features of this process are obscure. Here, we investigate whether activation of S100P/RAGE signaling regulates oncogenic microRNA-21 (miR-21). We show that exogenous S100P up-regulates miR-21 levels in human colon cancer cells, whereas knockdown of S100P results in a decrease of miR-21. Furthermore, blockage of RAGE with anti-RAGE antibody suppresses S100P induction of miR-21. In addition, we found that S100P induction of miR-21 expression involves ERK and is suppressed by the MEK inhibitor U0126. Also, S100P treatment stimulates the enrichment of c-Fos, and AP-1 family members, at the miR-21 gene promoter.

Original language	English (US)
Article number	37267
Pages (from-to)	2388-2393
Number of pages	6
Journal	FEBS Letters
Volume	589
Issue number	18
DOIs	https://doi.org/10.1016/j.febslet.2015.07.010
State	Published - Aug 12 2015

Keywords

AP-1
Colon cancer
Inflammation
Metastasis
RAGE
RECK
TCGA
miR-21
microRNAs

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2015.07.010

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title = "The S100P/RAGE signaling pathway regulates expression of microRNA-21 in colon cancer cells",

abstract = "Abstract S100P signaling through the receptor for advanced glycation end-products (RAGE) contributes to colon cancer invasion and metastasis, but the mechanistic features of this process are obscure. Here, we investigate whether activation of S100P/RAGE signaling regulates oncogenic microRNA-21 (miR-21). We show that exogenous S100P up-regulates miR-21 levels in human colon cancer cells, whereas knockdown of S100P results in a decrease of miR-21. Furthermore, blockage of RAGE with anti-RAGE antibody suppresses S100P induction of miR-21. In addition, we found that S100P induction of miR-21 expression involves ERK and is suppressed by the MEK inhibitor U0126. Also, S100P treatment stimulates the enrichment of c-Fos, and AP-1 family members, at the miR-21 gene promoter.",

keywords = "AP-1, Colon cancer, Inflammation, Metastasis, RAGE, RECK, TCGA, miR-21, microRNAs",

author = "Mercado-Pimentel, {Melania E.} and Onyeagucha, {Benjamin C.} and Qing Li and Pimentel, {Angel C.} and Jana Jandova and Nelson, {Mark A.}",

note = "Publisher Copyright: {\textcopyright} 2015 Federation of European Biochemical Societies.",

year = "2015",

month = aug,

day = "12",

doi = "10.1016/j.febslet.2015.07.010",

language = "English (US)",

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pages = "2388--2393",

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T1 - The S100P/RAGE signaling pathway regulates expression of microRNA-21 in colon cancer cells

AU - Mercado-Pimentel, Melania E.

AU - Onyeagucha, Benjamin C.

AU - Li, Qing

AU - Pimentel, Angel C.

AU - Jandova, Jana

AU - Nelson, Mark A.

PY - 2015/8/12

Y1 - 2015/8/12

N2 - Abstract S100P signaling through the receptor for advanced glycation end-products (RAGE) contributes to colon cancer invasion and metastasis, but the mechanistic features of this process are obscure. Here, we investigate whether activation of S100P/RAGE signaling regulates oncogenic microRNA-21 (miR-21). We show that exogenous S100P up-regulates miR-21 levels in human colon cancer cells, whereas knockdown of S100P results in a decrease of miR-21. Furthermore, blockage of RAGE with anti-RAGE antibody suppresses S100P induction of miR-21. In addition, we found that S100P induction of miR-21 expression involves ERK and is suppressed by the MEK inhibitor U0126. Also, S100P treatment stimulates the enrichment of c-Fos, and AP-1 family members, at the miR-21 gene promoter.

AB - Abstract S100P signaling through the receptor for advanced glycation end-products (RAGE) contributes to colon cancer invasion and metastasis, but the mechanistic features of this process are obscure. Here, we investigate whether activation of S100P/RAGE signaling regulates oncogenic microRNA-21 (miR-21). We show that exogenous S100P up-regulates miR-21 levels in human colon cancer cells, whereas knockdown of S100P results in a decrease of miR-21. Furthermore, blockage of RAGE with anti-RAGE antibody suppresses S100P induction of miR-21. In addition, we found that S100P induction of miR-21 expression involves ERK and is suppressed by the MEK inhibitor U0126. Also, S100P treatment stimulates the enrichment of c-Fos, and AP-1 family members, at the miR-21 gene promoter.

KW - AP-1

KW - Colon cancer

KW - Inflammation

KW - Metastasis

KW - RAGE

KW - RECK

KW - TCGA

KW - miR-21

KW - microRNAs

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JO - FEBS Letters

JF - FEBS Letters

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ER -

The S100P/RAGE signaling pathway regulates expression of microRNA-21 in colon cancer cells

Abstract

Keywords

ASJC Scopus subject areas

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