The role of thecal androgen production in the regulation of estradiol biosynthesis by dominant bovine follicles during the first follicular wave

K. E. Valdez, S. P. Cuneo, P. J. Gorden, A. M. Turzillo

    Research output: Contribution to journalArticlepeer-review

    18 Scopus citations

    Abstract

    The first wave of follicular development following ovulation in cattle is characterized by selection and growth of a large, estrogenic dominant follicle. After the follicle becomes morphologically dominant, concentrations of estradiol in its follicular fluid decrease abruptly. The purpose of this study was to determine whether this decrease in estrogen production is caused by an insufficient supply of androgen from theca interna or decreased aromatization of androgen precursor by granulosa cells. Dominant follicles were collected from Holstein heifers on d 4, 6, or 8 of the first follicular wave (n = 5/d). Amounts of 17α-hydroxylase mRNA in theca interna were sevenfold higher (P < 0.01) on d 4 than on d 8. After 3 h in culture, secretion of androstenedione by theca interna collected on d 4 (236 ± 44 pg/μg of protein) tended to be lower (P = 0.055) compared with d 6 (517 ± 162 pg/μg protein) and was lower (P < 0.05) compared with d 8 (387 ± 51 pg/μg of protein). In granulosa cells, amounts of aromatase mRNA decreased (P < 0.05) on d 8 compared with d 6 but not d 4. In vitro secretion of estradiol was higher in granulosa cells collected on d 4 (3.5 ± 0.8 ng/[105 cells × 3 h]) compared with d 6 (1.8 ± 0.6 ng/[105 cells × 3 h]; P < 0.05) and tended to be higher on d 4 than on d 8 (2.2 ± 0.2 ng/[105 cells × 3 h]; P = 0.058). We conclude that the decrease in estradiol production observed during atresia of the dominant follicle is not due to lack of androgen substrate for aromatization or downregulated expression of the aromatase gene, but may be the direct result of decreased activity of the aromatase enzyme within granulosa cells.

    Original languageEnglish (US)
    Pages (from-to)597-603
    Number of pages7
    JournalJournal of animal science
    Volume83
    Issue number3
    DOIs
    StatePublished - 2005

    Keywords

    • Bovine
    • Granulosa Cells
    • Ovary
    • Steroidogenesis
    • Theca interna

    ASJC Scopus subject areas

    • Food Science
    • Animal Science and Zoology
    • Genetics

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