The role of the G protein γ2 subunit in opioid antinociception in mice

Keiko Hosohata; Jennifer K. Logan; Eva Varga; Thomas H. Burkey; Todd W. Vanderah; Frank Porreca; Victor J. Hruby; William R. Roeske; Henry I. Yamamura

doi:10.1016/S0014-2999(00)00132-1

The role of the G protein γ₂ subunit in opioid antinociception in mice

Keiko Hosohata
, Jennifer K. Logan
, Eva Varga
, Thomas H. Burkey
, Todd W. Vanderah
, Frank Porreca
, Victor J. Hruby
, William R. Roeske
, Henry I. Yamamura

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

We examined the role of the γ₂ subunit of G proteins (Gγ₂) in the antinociception produced by c[D-Pen², D-Pen⁵]enkephalin (DPDPE) in mice. DPDPE produced 84.0±9.0% antinociception in vehicle-treated mice. After intracerebroventricular (i.c.v.) treatment with an antisense phosphorothioate oligodeoxynucleotide to the Gγ₂ subunit, DPDPE-mediated antinociception decreased to 24.4±7.4%. The mismatch phosphorothioate oligodeoxynucleotide-treated mice showed 65.1±10.3% antinociception, while the missense phosphorothioate oligodeoxynucleotide-treated mice showed 76.4±23.6% antinociception by DPDPE. The reduction of analgesia in antisense phosphorothioate oligodeoxynucleotide-treated mice was significant in comparison with vehicle-treated (P<0.001), mismatch phosphorothioate oligodeoxynucleotide-treated (P<0.01) and missense phosphorothioate oligodeoxynucleotide-treated (P<0.05) mice. These results suggest that the G protein γ₂ subunit is involved in the transduction pathway leading to antinociception by DPDPE.

Original language	English (US)
Pages (from-to)	R9-R11
Journal	European Journal of Pharmacology
Volume	392
Issue number	3
DOIs	https://doi.org/10.1016/S0014-2999(00)00132-1
State	Published - Mar 31 2000

Keywords

Antinociception
DPDPE (c[D-Pen, D-Pen]enkephalin)
G protein γ subunit

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/S0014-2999(00)00132-1

Cite this

@article{f7803c0369a44548b0185f62e641c0ed,

title = "The role of the G protein γ2 subunit in opioid antinociception in mice",

abstract = "We examined the role of the γ2 subunit of G proteins (Gγ2) in the antinociception produced by c[D-Pen2, D-Pen5]enkephalin (DPDPE) in mice. DPDPE produced 84.0±9.0\% antinociception in vehicle-treated mice. After intracerebroventricular (i.c.v.) treatment with an antisense phosphorothioate oligodeoxynucleotide to the Gγ2 subunit, DPDPE-mediated antinociception decreased to 24.4±7.4\%. The mismatch phosphorothioate oligodeoxynucleotide-treated mice showed 65.1±10.3\% antinociception, while the missense phosphorothioate oligodeoxynucleotide-treated mice showed 76.4±23.6\% antinociception by DPDPE. The reduction of analgesia in antisense phosphorothioate oligodeoxynucleotide-treated mice was significant in comparison with vehicle-treated (P<0.001), mismatch phosphorothioate oligodeoxynucleotide-treated (P<0.01) and missense phosphorothioate oligodeoxynucleotide-treated (P<0.05) mice. These results suggest that the G protein γ2 subunit is involved in the transduction pathway leading to antinociception by DPDPE.",

keywords = "Antinociception, DPDPE (c[D-Pen, D-Pen]enkephalin), G protein γ subunit",

author = "Keiko Hosohata and Logan, \{Jennifer K.\} and Eva Varga and Burkey, \{Thomas H.\} and Vanderah, \{Todd W.\} and Frank Porreca and Hruby, \{Victor J.\} and Roeske, \{William R.\} and Yamamura, \{Henry I.\}",

note = "Funding Information: This research was supported by a grant from the National Institute of Drug Abuse (DA 06284).",

year = "2000",

month = mar,

day = "31",

doi = "10.1016/S0014-2999(00)00132-1",

language = "English (US)",

volume = "392",

pages = "R9--R11",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - The role of the G protein γ2 subunit in opioid antinociception in mice

AU - Hosohata, Keiko

AU - Logan, Jennifer K.

AU - Varga, Eva

AU - Burkey, Thomas H.

AU - Vanderah, Todd W.

AU - Porreca, Frank

AU - Hruby, Victor J.

AU - Roeske, William R.

AU - Yamamura, Henry I.

N1 - Funding Information: This research was supported by a grant from the National Institute of Drug Abuse (DA 06284).

PY - 2000/3/31

Y1 - 2000/3/31

N2 - We examined the role of the γ2 subunit of G proteins (Gγ2) in the antinociception produced by c[D-Pen2, D-Pen5]enkephalin (DPDPE) in mice. DPDPE produced 84.0±9.0% antinociception in vehicle-treated mice. After intracerebroventricular (i.c.v.) treatment with an antisense phosphorothioate oligodeoxynucleotide to the Gγ2 subunit, DPDPE-mediated antinociception decreased to 24.4±7.4%. The mismatch phosphorothioate oligodeoxynucleotide-treated mice showed 65.1±10.3% antinociception, while the missense phosphorothioate oligodeoxynucleotide-treated mice showed 76.4±23.6% antinociception by DPDPE. The reduction of analgesia in antisense phosphorothioate oligodeoxynucleotide-treated mice was significant in comparison with vehicle-treated (P<0.001), mismatch phosphorothioate oligodeoxynucleotide-treated (P<0.01) and missense phosphorothioate oligodeoxynucleotide-treated (P<0.05) mice. These results suggest that the G protein γ2 subunit is involved in the transduction pathway leading to antinociception by DPDPE.

AB - We examined the role of the γ2 subunit of G proteins (Gγ2) in the antinociception produced by c[D-Pen2, D-Pen5]enkephalin (DPDPE) in mice. DPDPE produced 84.0±9.0% antinociception in vehicle-treated mice. After intracerebroventricular (i.c.v.) treatment with an antisense phosphorothioate oligodeoxynucleotide to the Gγ2 subunit, DPDPE-mediated antinociception decreased to 24.4±7.4%. The mismatch phosphorothioate oligodeoxynucleotide-treated mice showed 65.1±10.3% antinociception, while the missense phosphorothioate oligodeoxynucleotide-treated mice showed 76.4±23.6% antinociception by DPDPE. The reduction of analgesia in antisense phosphorothioate oligodeoxynucleotide-treated mice was significant in comparison with vehicle-treated (P<0.001), mismatch phosphorothioate oligodeoxynucleotide-treated (P<0.01) and missense phosphorothioate oligodeoxynucleotide-treated (P<0.05) mice. These results suggest that the G protein γ2 subunit is involved in the transduction pathway leading to antinociception by DPDPE.

KW - Antinociception

KW - DPDPE (c[D-Pen, D-Pen]enkephalin)

KW - G protein γ subunit

UR - https://www.scopus.com/pages/publications/0034737766

UR - https://www.scopus.com/pages/publications/0034737766#tab=citedBy

U2 - 10.1016/S0014-2999(00)00132-1

DO - 10.1016/S0014-2999(00)00132-1

M3 - Article

C2 - 10762674

AN - SCOPUS:0034737766

SN - 0014-2999

VL - 392

SP - R9-R11

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 3

ER -