The role of the G protein γ2 subunit in opioid antinociception in mice

Keiko Hosohata; Jennifer K. Logan; Eva Varga; Thomas H. Burkey; Todd W. Vanderah; Frank Porreca; Victor J. Hruby; William R. Roeske; Henry I. Yamamura

doi:10.1016/S0014-2999(00)00132-1

The role of the G protein γ₂ subunit in opioid antinociception in mice

Keiko Hosohata, Jennifer K. Logan, Eva Varga, Thomas H. Burkey, Todd W. Vanderah, Frank Porreca, Victor J. Hruby, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

We examined the role of the γ₂ subunit of G proteins (Gγ₂) in the antinociception produced by c[D-Pen², D-Pen⁵]enkephalin (DPDPE) in mice. DPDPE produced 84.0±9.0% antinociception in vehicle-treated mice. After intracerebroventricular (i.c.v.) treatment with an antisense phosphorothioate oligodeoxynucleotide to the Gγ₂ subunit, DPDPE-mediated antinociception decreased to 24.4±7.4%. The mismatch phosphorothioate oligodeoxynucleotide-treated mice showed 65.1±10.3% antinociception, while the missense phosphorothioate oligodeoxynucleotide-treated mice showed 76.4±23.6% antinociception by DPDPE. The reduction of analgesia in antisense phosphorothioate oligodeoxynucleotide-treated mice was significant in comparison with vehicle-treated (P<0.001), mismatch phosphorothioate oligodeoxynucleotide-treated (P<0.01) and missense phosphorothioate oligodeoxynucleotide-treated (P<0.05) mice. These results suggest that the G protein γ₂ subunit is involved in the transduction pathway leading to antinociception by DPDPE.

Original language	English (US)
Pages (from-to)	R9-R11
Journal	European Journal of Pharmacology
Volume	392
Issue number	3
DOIs	https://doi.org/10.1016/S0014-2999(00)00132-1
State	Published - Mar 31 2000

Keywords

Antinociception
DPDPE (c[D-Pen, D-Pen]enkephalin)
G protein γ subunit

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/S0014-2999(00)00132-1

Cite this

@article{f7803c0369a44548b0185f62e641c0ed,

title = "The role of the G protein γ2 subunit in opioid antinociception in mice",

abstract = "We examined the role of the γ2 subunit of G proteins (Gγ2) in the antinociception produced by c[D-Pen2, D-Pen5]enkephalin (DPDPE) in mice. DPDPE produced 84.0±9.0% antinociception in vehicle-treated mice. After intracerebroventricular (i.c.v.) treatment with an antisense phosphorothioate oligodeoxynucleotide to the Gγ2 subunit, DPDPE-mediated antinociception decreased to 24.4±7.4%. The mismatch phosphorothioate oligodeoxynucleotide-treated mice showed 65.1±10.3% antinociception, while the missense phosphorothioate oligodeoxynucleotide-treated mice showed 76.4±23.6% antinociception by DPDPE. The reduction of analgesia in antisense phosphorothioate oligodeoxynucleotide-treated mice was significant in comparison with vehicle-treated (P<0.001), mismatch phosphorothioate oligodeoxynucleotide-treated (P<0.01) and missense phosphorothioate oligodeoxynucleotide-treated (P<0.05) mice. These results suggest that the G protein γ2 subunit is involved in the transduction pathway leading to antinociception by DPDPE.",

keywords = "Antinociception, DPDPE (c[D-Pen, D-Pen]enkephalin), G protein γ subunit",

author = "Keiko Hosohata and Logan, {Jennifer K.} and Eva Varga and Burkey, {Thomas H.} and Vanderah, {Todd W.} and Frank Porreca and Hruby, {Victor J.} and Roeske, {William R.} and Yamamura, {Henry I.}",

note = "Funding Information: This research was supported by a grant from the National Institute of Drug Abuse (DA 06284).",

year = "2000",

month = mar,

day = "31",

doi = "10.1016/S0014-2999(00)00132-1",

language = "English (US)",

volume = "392",

pages = "R9--R11",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - The role of the G protein γ2 subunit in opioid antinociception in mice

AU - Hosohata, Keiko

AU - Logan, Jennifer K.

AU - Varga, Eva

AU - Burkey, Thomas H.

AU - Vanderah, Todd W.

AU - Porreca, Frank

AU - Hruby, Victor J.

AU - Roeske, William R.

AU - Yamamura, Henry I.

N1 - Funding Information: This research was supported by a grant from the National Institute of Drug Abuse (DA 06284).

PY - 2000/3/31

Y1 - 2000/3/31

N2 - We examined the role of the γ2 subunit of G proteins (Gγ2) in the antinociception produced by c[D-Pen2, D-Pen5]enkephalin (DPDPE) in mice. DPDPE produced 84.0±9.0% antinociception in vehicle-treated mice. After intracerebroventricular (i.c.v.) treatment with an antisense phosphorothioate oligodeoxynucleotide to the Gγ2 subunit, DPDPE-mediated antinociception decreased to 24.4±7.4%. The mismatch phosphorothioate oligodeoxynucleotide-treated mice showed 65.1±10.3% antinociception, while the missense phosphorothioate oligodeoxynucleotide-treated mice showed 76.4±23.6% antinociception by DPDPE. The reduction of analgesia in antisense phosphorothioate oligodeoxynucleotide-treated mice was significant in comparison with vehicle-treated (P<0.001), mismatch phosphorothioate oligodeoxynucleotide-treated (P<0.01) and missense phosphorothioate oligodeoxynucleotide-treated (P<0.05) mice. These results suggest that the G protein γ2 subunit is involved in the transduction pathway leading to antinociception by DPDPE.

AB - We examined the role of the γ2 subunit of G proteins (Gγ2) in the antinociception produced by c[D-Pen2, D-Pen5]enkephalin (DPDPE) in mice. DPDPE produced 84.0±9.0% antinociception in vehicle-treated mice. After intracerebroventricular (i.c.v.) treatment with an antisense phosphorothioate oligodeoxynucleotide to the Gγ2 subunit, DPDPE-mediated antinociception decreased to 24.4±7.4%. The mismatch phosphorothioate oligodeoxynucleotide-treated mice showed 65.1±10.3% antinociception, while the missense phosphorothioate oligodeoxynucleotide-treated mice showed 76.4±23.6% antinociception by DPDPE. The reduction of analgesia in antisense phosphorothioate oligodeoxynucleotide-treated mice was significant in comparison with vehicle-treated (P<0.001), mismatch phosphorothioate oligodeoxynucleotide-treated (P<0.01) and missense phosphorothioate oligodeoxynucleotide-treated (P<0.05) mice. These results suggest that the G protein γ2 subunit is involved in the transduction pathway leading to antinociception by DPDPE.

KW - Antinociception

KW - DPDPE (c[D-Pen, D-Pen]enkephalin)

KW - G protein γ subunit

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JF - European Journal of Pharmacology

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