TY - JOUR
T1 - The response of renal tubular epithelial cells to physiologically and chemically induced growth arrest
AU - Jeong, Jeongmi K.
AU - Huang, Qihong
AU - Lau, Serrine S.
AU - Monks, Terrence J.
PY - 1997/3/14
Y1 - 1997/3/14
N2 - Cells respond to a variety of stresses by activating the transcription of a battery of 'acute phase' or 'stress response' genes. The nature of this response is tailored to the nature of the stress. The extent to which physiologically and pathophysiologically induced growth arrest share common genomic responses is unclear. We therefore compared the effects of a physiologically induced (serum and nutrient depletion) and a chemically induced (2-Br-bis-(GSyl)HQ and 2-Br-6-(GSyl)HQ) stress in renal tubular epithelial cells (LLC-PK1). The response to physiological stress, induced by serum depletion, involves growth arrest characterized by an inhibition of DNA synthesis that occurs in the absence of a decrease in histone mRNA or an increase in gadd153 mRNA, one of the growth arrest and DNA damage inducible genes. In contrast, the chemical-induced stress involves growth arrest accompanied by a decrease in histone mRNA, particularly core histone H2B and H2A mRNA, and the induction of gadd153. Chemical-induced changes in histone mRNA inversely correlate to changes in the expression of a stress gene, hsp70, whose expression is dependent upon the maintenance of appropriate nucleosomal structure.
AB - Cells respond to a variety of stresses by activating the transcription of a battery of 'acute phase' or 'stress response' genes. The nature of this response is tailored to the nature of the stress. The extent to which physiologically and pathophysiologically induced growth arrest share common genomic responses is unclear. We therefore compared the effects of a physiologically induced (serum and nutrient depletion) and a chemically induced (2-Br-bis-(GSyl)HQ and 2-Br-6-(GSyl)HQ) stress in renal tubular epithelial cells (LLC-PK1). The response to physiological stress, induced by serum depletion, involves growth arrest characterized by an inhibition of DNA synthesis that occurs in the absence of a decrease in histone mRNA or an increase in gadd153 mRNA, one of the growth arrest and DNA damage inducible genes. In contrast, the chemical-induced stress involves growth arrest accompanied by a decrease in histone mRNA, particularly core histone H2B and H2A mRNA, and the induction of gadd153. Chemical-induced changes in histone mRNA inversely correlate to changes in the expression of a stress gene, hsp70, whose expression is dependent upon the maintenance of appropriate nucleosomal structure.
UR - http://www.scopus.com/inward/record.url?scp=0030986142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030986142&partnerID=8YFLogxK
U2 - 10.1074/jbc.272.11.7511
DO - 10.1074/jbc.272.11.7511
M3 - Article
C2 - 9054455
AN - SCOPUS:0030986142
SN - 0021-9258
VL - 272
SP - 7511
EP - 7518
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 11
ER -