The pre-mRNA splicing reaction

Somsakul Pop Wongpalee, Shalini Sharma

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


In eukaryotic organisms, nascent transcripts of protein-coding genes contain intronic sequences that are not present in mature mRNAs. Pre-mRNA splicing removes introns and joins exons to form mature mRNAs. It is catalyzed by a large RNP complex called the spliceosome. Sequences within the pre-mRNA determine intron recognition and excision. This process occurs with a high degree of accuracy to generate the functional transcriptome of a cell.

Original languageEnglish (US)
Title of host publicationSpliceosomal Pre-mRNA Splicing
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages10
ISBN (Print)9781627039796
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Exon
  • Intron
  • Intronless
  • Pre-mRNA
  • Splice site
  • Spliceosome
  • Splicing
  • Transcript

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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