TY - JOUR
T1 - The physical and genetic framework of the maize B73 genome
AU - Wei, Fusheng
AU - Zhang, Jianwei
AU - Zhou, Shiguo
AU - He, Ruifeng
AU - Schaeffer, Mary
AU - Collura, Kristi
AU - Kudrna, David
AU - Faga, Ben P.
AU - Wissotski, Marina
AU - Golser, Wolfgang
AU - Rock, Susan M.
AU - Graves, Tina A.
AU - Fulton, Robert S.
AU - Coe, Ed
AU - Schnable, Patrick S.
AU - Schwartz, David C.
AU - Ware, Doreen
AU - Clifton, Sandra W.
AU - Wilson, Richard K.
AU - Wing, Rod A.
PY - 2009/11
Y1 - 2009/11
N2 - Maize is a major cereal crop and an important model system for basic biological research. Knowledge gained from maize research can also be used to genetically improve its grass relatives such as sorghum, wheat, and rice. The primary objective of the Maize Genome Sequencing Consortium (MGSC) was to generate a reference genome sequence that was integrated with both the physical and genetic maps. Using a previously published integrated genetic and physical map, combined with in-coming maize genomic sequence, new sequence-based genetic markers, and an optical map, we dynamically picked a minimum tiling path (MTP) of 16,910 bacterial artificial chromosome (BAC) and fosmid clones that were used by the MGSC to sequence the maize genome. The final MTP resulted in a significantly improved physical map that reduced the number of contigs from 721 to 435, incorporated a total of 8,315 mapped markers, and ordered and oriented the majority of FPC contigs. The new integrated physical and genetic map covered 2,120 Mb (93%) of the 2,300-Mb genome, of which 405 contigs were anchored to the genetic map, totaling 2,103.4 Mb (99.2% of the 2,120 Mb physical map). More importantly, 336 contigs, comprising 94.0% of the physical map (∼1,993 Mb), were ordered and oriented. Finally we used all available physical, sequence, genetic, and optical data to generate a golden path (AGP) of chromosome-based pseudomolecules, herein referred to as the B73 Reference Genome Sequence version 1 (B73 RefGen-v1).
AB - Maize is a major cereal crop and an important model system for basic biological research. Knowledge gained from maize research can also be used to genetically improve its grass relatives such as sorghum, wheat, and rice. The primary objective of the Maize Genome Sequencing Consortium (MGSC) was to generate a reference genome sequence that was integrated with both the physical and genetic maps. Using a previously published integrated genetic and physical map, combined with in-coming maize genomic sequence, new sequence-based genetic markers, and an optical map, we dynamically picked a minimum tiling path (MTP) of 16,910 bacterial artificial chromosome (BAC) and fosmid clones that were used by the MGSC to sequence the maize genome. The final MTP resulted in a significantly improved physical map that reduced the number of contigs from 721 to 435, incorporated a total of 8,315 mapped markers, and ordered and oriented the majority of FPC contigs. The new integrated physical and genetic map covered 2,120 Mb (93%) of the 2,300-Mb genome, of which 405 contigs were anchored to the genetic map, totaling 2,103.4 Mb (99.2% of the 2,120 Mb physical map). More importantly, 336 contigs, comprising 94.0% of the physical map (∼1,993 Mb), were ordered and oriented. Finally we used all available physical, sequence, genetic, and optical data to generate a golden path (AGP) of chromosome-based pseudomolecules, herein referred to as the B73 Reference Genome Sequence version 1 (B73 RefGen-v1).
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U2 - 10.1371/journal.pgen.1000715
DO - 10.1371/journal.pgen.1000715
M3 - Article
C2 - 19936061
AN - SCOPUS:73649087948
SN - 1553-7390
VL - 5
JO - PLoS genetics
JF - PLoS genetics
IS - 11
M1 - e1000715
ER -