The ontogeny of specific binding sites for the calcium channel antagonist, nitrendipine, in mouse heart and brain

Russ D. Erman; Henry I. Yamamura; William R. Roeske

doi:10.1016/0006-8993(83)90265-2

The ontogeny of specific binding sites for the calcium channel antagonist, nitrendipine, in mouse heart and brain

Russ D. Erman, Henry I. Yamamura, William R. Roeske

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The high specific activity radioligand, [³H]nitrendipine, was used to study the ontogeny of high affinity binding sites in mouse heart and brain. A period of rapid postnatal brain growth is reflected by a dramatic increase in the receptor density (B_max) between 7 and 21 days of age, reaching an adult B_max value of 10.7 ± 0.38 fmol/mg tissue. A more linear rise in cardiac binding site density to 16.8 ± 2.0 fmol/mg tissue was found while no change in K_d of either tissue was noted during development.

Original language	English (US)
Pages (from-to)	327-331
Number of pages	5
Journal	Brain Research
Volume	278
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(83)90265-2
State	Published - Nov 14 1983

Keywords

brain
calcium channels
heart
mice
nitrendipine
ontogeny

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(83)90265-2

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title = "The ontogeny of specific binding sites for the calcium channel antagonist, nitrendipine, in mouse heart and brain",

abstract = "The high specific activity radioligand, [3H]nitrendipine, was used to study the ontogeny of high affinity binding sites in mouse heart and brain. A period of rapid postnatal brain growth is reflected by a dramatic increase in the receptor density (Bmax) between 7 and 21 days of age, reaching an adult Bmax value of 10.7 ± 0.38 fmol/mg tissue. A more linear rise in cardiac binding site density to 16.8 ± 2.0 fmol/mg tissue was found while no change in Kd of either tissue was noted during development.",

keywords = "brain, calcium channels, heart, mice, nitrendipine, ontogeny",

author = "Erman, {Russ D.} and Yamamura, {Henry I.} and Roeske, {William R.}",

note = "Funding Information: Special thanks are extended to Jane Grewling and Linda Grunwald for technical aid, Terry Austin for secretarial assistance, and Susan Yamamura for provision of the analytical computer program. Portions of this study were supported by USHS Grants MH-30626, MH-27257, HL-29565, and Program Project Grant HL 20984, a Research Scientist Development Award Type II (MH-00095) from the National Institute of Mental Health to H.I.Y., and a Research Career Development Award (HL-00776) from the National Heart, Lung and Blood Institute to W.R.R.",

year = "1983",

month = nov,

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doi = "10.1016/0006-8993(83)90265-2",

language = "English (US)",

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T1 - The ontogeny of specific binding sites for the calcium channel antagonist, nitrendipine, in mouse heart and brain

AU - Erman, Russ D.

AU - Yamamura, Henry I.

AU - Roeske, William R.

N1 - Funding Information: Special thanks are extended to Jane Grewling and Linda Grunwald for technical aid, Terry Austin for secretarial assistance, and Susan Yamamura for provision of the analytical computer program. Portions of this study were supported by USHS Grants MH-30626, MH-27257, HL-29565, and Program Project Grant HL 20984, a Research Scientist Development Award Type II (MH-00095) from the National Institute of Mental Health to H.I.Y., and a Research Career Development Award (HL-00776) from the National Heart, Lung and Blood Institute to W.R.R.

PY - 1983/11/14

Y1 - 1983/11/14

N2 - The high specific activity radioligand, [3H]nitrendipine, was used to study the ontogeny of high affinity binding sites in mouse heart and brain. A period of rapid postnatal brain growth is reflected by a dramatic increase in the receptor density (Bmax) between 7 and 21 days of age, reaching an adult Bmax value of 10.7 ± 0.38 fmol/mg tissue. A more linear rise in cardiac binding site density to 16.8 ± 2.0 fmol/mg tissue was found while no change in Kd of either tissue was noted during development.

AB - The high specific activity radioligand, [3H]nitrendipine, was used to study the ontogeny of high affinity binding sites in mouse heart and brain. A period of rapid postnatal brain growth is reflected by a dramatic increase in the receptor density (Bmax) between 7 and 21 days of age, reaching an adult Bmax value of 10.7 ± 0.38 fmol/mg tissue. A more linear rise in cardiac binding site density to 16.8 ± 2.0 fmol/mg tissue was found while no change in Kd of either tissue was noted during development.

KW - brain

KW - calcium channels

KW - heart

KW - mice

KW - nitrendipine

KW - ontogeny

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ER -

The ontogeny of specific binding sites for the calcium channel antagonist, nitrendipine, in mouse heart and brain

Abstract

Keywords

ASJC Scopus subject areas

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